Apoptotic protease activating factor 1, also known as APAF1, is a human homolog of C. elegans CED-4 gene. [5][6][7] The protein was identified in the laboratory of Xiaodong Wang as an activator of caspase-3 in the presence of cytochromeC and dATP. [8] .

Apoptotic protease activating factor-1 (Apaf-1) is a key molecule in the intrinsic or mitochondrial pathway of apoptosis, which oligomerizes in response to cytochrome c release and forms a large complex known as apoptosome.

2025年1月21日 · Apoptotic protease activating factor 1 (Apaf-1) was traditionally defined as a scaffold protein in mammalian cells for assembling a caspase activation platform known as the ‘apoptosome’...

2017年4月1日 · Apoptotic protease activating factor-1 (Apaf-1) is a key molecule in the intrinsic or mitochondrial pathway of apoptosis, which oligomerizes in response to cytochrome c release and forms a large complex known as apoptosome.

Apaf-1 is a multidomain adapter protein containing an N-terminal caspase recruitment domain (CARD), followed by a nucleotide-binding and oligomerization domain (NOD, also known as NB-ARC) that is homologous to CED-4 in Caenorhabditis elegans, and a C-terminal regulatory Y-domain composed of 12–13 WD40 repeats (WDRs), which form seven- and six ...

2021年3月9日 · The authors surprisingly identified Apaf-1, the key adaptor of intrinsic apoptosis, interacted with caspase-4 directly, and they named this protein complex as Apaf-1 pyroptosome.

2025年1月21日 · Apoptotic protease activating factor 1 (Apaf-1) was traditionally defined as a scaffold protein in mammalian cells for assembling a caspase activation platform known as the 'apoptosome' after its binding to cytochrome c.

2017年12月24日 · APAF1 (Apoptotic Peptidase Activating Factor 1) is a Protein Coding gene. Diseases associated with APAF1 include Autism Spectrum Disorder and Transitional Cell Carcinoma. Among its related pathways are TNFR1 Pathway and Apoptosis and survival FAS signaling cascades.

2002年2月1日 · In the case of the latter complex, the central scaffold protein is a mammalian CED-4 homologue known as Apaf-1. This is an ∼130 kDa protein, which in the presence of cytochrome c and dATP oligomerizes to form a very large …

1997年8月8日 · We report here the purification and cDNA cloning of Apaf-1, a novel 130 kd protein from HeLa cell cytosol that participates in the cytochrome c–dependent activation of caspase-3. The NH2-terminal 85 amino acids of Apaf-1 show 21% identity and 53% similarity to the NH2-terminal prodomain of the Caenorhabditis elegans caspase, CED-3.