2022年1月2日 · Stepwise immobilization promotes uniform orientation of bound IgGs onto the immunosensor. Heterofunctional matrices are more cost-effective immuno-platform with great orientation controllability. Exploitation of site-oriented full-length IgGs provide more option for immunosensing.

2018年1月1日 · The immobilization of antibodies on sensing platforms is the first critical step in the development of immunoassays with excellent bioanalytical performance. The ideal immobilization strategy should be leach-proof, rapid, and less labor-intensive to provide high detection sensitivity and specificity without the requirement of high-end skillset ...

2017年3月16日 · This review describes the most recent methods for oriented antibody immobilization and the characterization techniques employed for investigation of the antibody state. The introduction describes the importance of …

2017年8月28日 · We studied the dependencies of covalent antibody immobilization using the hetero-crosslinker 1-ethyl-3- (3-dimethylaminopropyl)carbodiimide (EDC), which provides a stable bond between a carboxyl group on one end and a primary amine on the other end.

In this review, we are discussing antibody immobilization strategies that provide (i) site-specific capture guided through various tags and functional groups on antibodies and (ii) orientation achieved through pre-capture biomolecules.

2023年2月17日 · In this chapter, we discuss the various approaches and methods leading to successful immobilization of antibody to a solid support. The chapter begins with a description of the solid support surface chemistries and strategies commonly used for …

2022年2月28日 · In highly sensitive detection systems, the antibodies are generally immobilized on the solid supports of the sensing platforms, and antigens are detected by monitoring the signals resulting from the binding between antibodies and antigens.

2017年3月1日 · Immobilized antibody systems are the key to develop efficient diagnostics and separations tools. In the last decade, developments in the field of biomolecular engineering and crosslinker chemistry have greatly influenced the development of this field.

2025年2月13日 · Antibodies that target the Plasmodium falciparum histidine-rich protein-II biomarker (PfHRP-II) are being used in rapid diagnostic tests (RDTs) of malaria. PfHRP-II levels associated with severe malaria are typically greater than 100 ng mL–1. Unfortunately, genetic variations within the PfHRP-II gene can reduce the reliability of these RDTs by affecting both …

A review of the sites (primary amines, sulfhydryls, carbohydrate moieties) on antibodies that are available for labeling with enzymes, fluorophores or biotin, or for immobilization to solid support.