2017年7月21日 · New methods control errors sufficiently such that amplicon sequence variants (ASVs) can be resolved exactly, down to the level of single-nucleotide differences over the sequenced gene region.

Amplicon Sequence Variants (ASVs) have become a widely adopted high-resolution technique in microbiome research. In contrast to conventional Operational Taxonomic Unit (OTU)-based methods, ASVs detect sequence variations at a single-nucleotide level, enabling enhanced precision and reliability.

2024年7月27日 · Amplicon sequencing allows the efficient identification and screening of genetic variants. Amplicon sequencing can support multiplexing multiple amplicons per reaction which ensures high coverage of the targeted regions.

“the use of exact sequences instead of clustered operational taxonomic units, enable bacterial and archaeal ribosomal RNA gene sequences to be followed across multiple studies and allow us to explore patterns of diversity at an unprecedented scale…for many sample types, especially plant-associated and free-living

2021年7月21日 · As an alternative to operational taxonomic units (OTUs), amplicon sequencing variants (ASVs) have been proposed as a way to adopt the thresholds suggested by genome sequencing to microbial community analysis using 16S rRNA gene sequences (7 – 10).

For example, there was a proposal to replace operational taxonomic units (OTUs) with amplicon sequence variants (ASVs) in marker gene-based amplicon data analysis (Callahan et al., 2016).

2023年7月21日 · Successful implementation of a naïve Bayesian classifier for cpn60 sequences will facilitate future microbiome studies and open opportunities to integrate cpn60 amplicon sequence...

Inferring Amplicon Sequence Variants (ASVs) •So far, we have assigned p-values for each sequence in each sample •DADA2 then tries to determine which sequences are of biological origin (ASVs) and which aren’t by assessing which sequences are present in other samples •If a sequence is present in another sample, it is more likely that it is a

2024年6月5日 · In amplicon sequencing a particular genetic locus, for example the 16S rRNA gene (or a part of it) in bacteria, is amplified from DNA extracted from the community of interest, and then sequenced on a next-generation sequencing platform.

2023年9月14日 · When it comes to taxonomic profiling of the microbiome, amplicon sequence variants (ASVs) is a method that has unique benefits compared to the traditional method of sequence similarity-based operational taxonomic unit (OTU) clustering.