Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering.

2024年9月25日 · Acute fatty liver of pregnancy (AFLP) is an obstetric emergency characterized by maternal liver dysfunction and/or failure that can lead to maternal and fetal complications, including death. Prompt delivery and supportive maternal care are important for achieving a full recovery for the mother.

2017年11月8日 · The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA. How It Works. After final amplification, selectively amplified fragments are separated by gel electrophoresis and visualized autoradiographically.

Amplified fragment length polymorphism (AFLP) is a PCR-based technique that uses selective amplification of a subset of digested DNA fragments to generate and compare unique fingerprints for genomes of interest.

AFLP is a highly sensitive method for detecting polymorphism in DNA. AFLP is a dominant type of molecular marker, it involves restriction followed by PCR amplification of genomic DNA. Primers and adapters are used for pre-selective and selective PCR amplifications.

Amplified Fragment Length Polymorphism is a nucleic acid finger printing method to find out genetic variation that exists between closely related genes. This method was de­signed by Zabeau and Vos (1993) and Vos et al. (1995).

AFLP is a versatile technique for genome-wide screening of genetic diversity and can be applied to almost any organism. The technique relies on detecting genetic polymorphisms through differential endonuclease restriction digestion of genomic DNA.