In vector plasmids, ITR sequences were assessed with multiple restriction enzymes to confirm the presence of the ITRs and their genotype identity. Initially, the activity of ITR2 was compared with the “strong” CBA promoter. AAV2-ITR-luciferase and AAV2-CBA-luciferase were packaged into AAV2 capsids and used to infect HEK293 cells at 1E5 vg ...

2023年10月19日 · ITRs are symmetrical 145-nucleotide sequences that flank the ends of the single stranded DNA genome of AAV in the native virus. In an AAV vector plasmid, they flank the gene of interest. A single ITR consists of palindromic arms (A-A’, B-B’, and C-C’) that give the feature its characteristic T-shape.

Eleven serotypes of AAV have thus far been identified, with the best characterized and most commonly used being AAV2. These serotypes differ in their tropism, or the types of cells they infect, making AAV a very useful system for preferentially transducing specific cell types.

The AAV2 ITR (Inverted Terminal Repeat) sequence is a specific nucleotide sequence found in the Adeno-Associated Virus serotype 2. It plays a critical role in the virus's life cycle,...

In the present study, we sought to evaluate the mechanisms by which inverted terminal repeat (ITR) sequences mediate intermolecular recombination of AAV genomes, with the goal of engineering more efficient vectors for dual-vector trans-splicing approaches.

2020年8月11日 · ITR sequences are 145 bases each and AAV plasmids have two ITR sequences. The DNA sequence between the ITRs is what gets packaged into the AAV molecule. What’s outside of the ITRs does not get packaged in the AAV vector.

2024年2月1日 · The primary obstacle to the detection of ITR-containing plasmids and rAAV genomes stems from the complex nature of the ITR sequence, characterized by an exceptionally high GC base content (70%) and its palindromic structure.

2021年10月6日 · To study whether synthetic ITRs are suitable for AAV gene therapy, we substituted ~15% of the nucleotides in the AAV2 ITR, the most used ITR in AAV vectors. Specifically, we targeted the CpG...

Inverted terminal repeat (ITR) integrity is critical for the replication, packaging, and transduction of recombinant adeno-associated virus (rAAV), a promising gene therapy vector. Because AAV...

Sequence Analyzer. Analyze a DNA sequence to see restriction sites and map. Vector Database. Browse a digital-only collection of vector backbone information