An initial screening of the inhibition potential of CBD and 7-OH-CBD against individual CYP and FMO enzymes was determined using microsomes (30–100 μg) from CYP- or FMO-overexpressing HEK293 cell lines in reactions containing either 1 or 10 μM CBD or 7-OH-CBD as potential inhibitors, nicotine or cotinine as substrates, 100 mM potassium ...

2022年1月25日 · Next, 7-COOH-CBD is converted into various glucuronide conjugates via phase II liver metabolism before being excreted via the urine and feces. Most of the phase II metabolism of CBD is carried out by the UDP-glucuronosyltransferases UGT1A9 and UGT2B7 [ 14 ].

The first CBD metabolites to be identified were isolated from rat liver homogenate and their structures were determined as a primary alcohol derived from the oxidation at the allylic C-7 methyl group on the cyclohexene moiety (7-OH-CBD) and a secondary alcohol resulting from the oxidation of the central (C-3′′) methylene group of the pentyl ...

2023年2月20日 · Inhibition (IC 50,u = 0.37 ± 0.06 μM) of 3HC to 3HC-glucuronide formation by UGT1A9 was demonstrated by CBD. Significant inhibition of nicotine metabolism pathways by CBD and 7-OH-CBD suggests that cannabinoids may inhibit nicotine metabolism, potentially impacting tobacco addiction and cessation.

2022年11月18日 · CBD is metabolized via CYPs 2C19, and 2C9 to an active metabolite 7-OH-CBD, which is further metabolized to the inactive metabolite CBD-COOH [Citation 102, Citation 103]. Like CBN, CBD also undergoes direct glucuronidation via UGTs 1A9, 2B7, and 2B17 [ …

7-hydroxycannabidiol is a hydroxy - cannabidiol that is cannabidiol in which the methyl group that is attached to the double bond of the cyclohexene ring has been oxidised to the corresponding hydroxymethyl group. It is one of the main metabolites of cannabidiol by human liver microsomes, produced particularly by CYP2C19.

Glucuronide conjugates of cannabidiol (CBD), 7-hydroxy-CBD, propyl-CBD, cannabinol (CBN), 7-hydroxy-CBN, CBN-7-oic acid, propyl CBN and cannabichromene have been identified as major metabolites of CBD, CBN and their propyl homologues and of cannabichromene in mouse liver. Trace amounts of the glucur …

2016年7月1日 · Identifying recent cannabis intake is confounded by prolonged cannabinoid excretion in chronic frequent cannabis users. We previously observed detection times ≤2.1h for cannabidiol (CBD) and cannabinol (CBN) and Δ(9)-tetrahydrocannabinol (THC)-glucuronide in whole blood after smoking, suggesting the …

Significant inhibition of nicotine metabolism pathways by CBD and 7-OH-CBD suggests that cannabinoids may inhibit nicotine metabolism, potentially impacting tobacco addiction and cessation.

Phase II metabolism by UDP-glucuronosyltransferases yields more hydrophilic metabolites including CBD-6-OH-CBD- and 7-OH-CBD-glucuronides [19 – 21]. There are few pharmacokinetic data on urinary CBD excretion.