Characteristics of FreeStyle™ 293-F cells: • Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were re-cloned by limiting dilution. The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages.

293F细胞是一类能够在无血清中高表达蛋白的野生型293细胞系。 293FT细胞则是在293F细胞系中插入pCMVSPORT6TAg.Neo 质粒的转化细胞系，能够快速增值，易转染，其能够用于慢病毒载体的生产。 293FTM细胞则是来源于 Flp-InTMT-RETM293细胞，该细胞系中转染了ecotropic receptor质粒及MAPPIT (mammalian protein-protein interaction trap)报告质粒，该细胞系主要 …

Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium.

The FreeStyleTM 293-F cell line is derived from the 293 cell line (see “Parental cell line“ on page 6) and is intended for use with the FreeStyleTM MAX 293 Expression System (Cat. no. K9000-10) or the FreeStyleTM 293 Expression System (Cat. no. K9000-01).

FreestyleTM 293-F cells from Life Technologies are prone to clumping at higher densities and volumes. Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc., cat. No. 23966) is prepared as a stock solution at a concentration of 1 mg/ml in a buffer containing 25mM HEPES and 150 mM NaCl (pH 7.5).

293-F cells are GIBCO brand cells. These are clonal isolates derived from transformed human embryonal kidney (HEK) cells. The 293-F strain is a fast-growing variant of the 293 cell line. They have been adapted to suspension culture in serum-free media, 293 SFM II.

Human Embryonic Kidney (HEK) cell line (e.g., Freestyle HEK 293F cells). These cells have been derived from the HEK 293 cell line, are adapted to grow in suspension cultures, reaching high ...

293f细胞以其高效的蛋白表达能力著称，能够在无血清培养基中快速生长并表达高水平的重组蛋白。 293F细胞具有高转染效率，适合用于重组蛋白和病毒载体的生产。

赛默飞 FreeStyle 293-F 细胞是一种完整的悬浮细胞培养系统，可生成大量哺乳动物重组蛋白。 简化实验室操作，减少播种、喂养和传代的时间和劳动强度。 适用于各种生物研究和生产需求。 立即了解更多，提升实验效率！

Gibco™ 293-F cells were prepared from a clone selected for fast growth in serum-free medium, superior transfection efficiencies and a high level of protein expression. Optimal transfection efficiencies are obtained with Lipofectamine™ 2000 or 293fectin™ transfection reagents.