2021年10月26日 · Here, we chemically synthesize a panel of homogenous ubiquitinated proteins, and use them to compare 20S and 26S proteasomes with respect to substrate selection and peptide-product generation. We...

2024年9月18日 · New proteasomes are produced to accommodate increases in cellular catabolic demand and prevent the accumulation of cytotoxic proteins. Formation of the proteasomal 20S core complex relies on the...

The 20S (700-kDa) proteasome contains multiple peptidase activities that function through a new type of proteolytic mechanism involving a threonine active site. The 26S (2000-kDa) complex, which degrades ubiquitinated proteins, contains in addition to the 20S proteasome a 19S regulatory complex composed of multiple ATPases and components ...

Peptidomics identifies proteasome-trapped ubiquitin-derived peptides and peptides of potential 20S substrates in Hi20S cells, hypoxic cells, and human failing-heart. Moreover, elevated levels of 20S proteasomes appear to contribute to cell survival under stress associated with …

2025年1月10日 · Here, we captured endogenous maturation intermediates of human 20S proteasome, which are low abundance and highly dynamic, and determined their structures by cryo–electron microscopy. Through structure-based functional studies, we identified the key switches that remodel and activate the proteolytic sites.

The 20S proteasome cleaves proteins that contain partially unfolded regions; thus, capable of entering into its catalytic chamber. Two main groups of substrates that fall into this category were shown to be susceptible to 20S proteolysis.

Biogenesis of the eukaryotic 20S proteasome relies on the intrinsic ability of subunits to self-assemble, subunit-specific N-terminal and C-terminal extensions that help guide assembly, and dedicated extrinsic assembly chaperones.

2019年5月16日 · Here, we will describe recent advances in our knowledge of the functional aspects of the 20S proteasome, exploring several different systems and processes. These include neuronal communication, post-translational processing, oxidative stress, intrinsically disordered protein regulation, and extracellular proteasomes.

2024年1月29日 · 20S proteasome substrates are primarily RNA- and DNA-binding proteins with intrinsically disordered regions. The 20S proteasome not only fully degrades substrates but also cleaves proteins at their N- and C-termini, indicating regulatory actions. Oxidized proteasomes show lower proteolytic activity in comparison to naïve proteasomes.

2019年5月16日 · Here, we will describe recent advances in our knowledge of the functional aspects of the 20S proteasome, exploring several different systems and processes. These include neuronal communication, post-translational processing, oxidative stress, intrinsically disordered protein regulation, and extracellular proteasomes.