The Visium platform delivers unbiased, whole transcriptome spatial gene expression analysis at single cell scale with unmatched spatial data quality and offers a flexible assay portfolio to study a broad range of tissues.

Visium Spatial Gene Expression is a next-generation molecular profiling solution for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context in FFPE or fresh frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research.

Pair powerful whole transcriptome spatial gene expression with single cell–scale resolution to discover the unique biology in every micron of tissue. Obtain best-in-class data with the simple-to-run CytAssist-enabled workflow. Manufactured under license from Centrillion.

2020年3月24日 · Visium空间基因表达解决方案可测量完整组织切片中的总mRNA，并绘制出现基因活性的位置的图。 简单说，就是将基因的表达情况与切片染色图结合起来，在一个空间的维度上去理解基因表达的分布情况. 包括： 自己实验室需要配备： 出于组织学目的，对新鲜冷冻的组织切片进行成像，并将其放置在包含与RNA结合的捕获探针的阵列上。 将组织固定并透化以释 …

Generally, Visium HD performance exceeds or is comparable to Visium v2 when sensitivity is calculated on a per-tissue area basis and both assays are sequenced at the same relative depth (see the table below for additional comparisons).

We offer ALL 10x Genomics protocols, including the original Fresh Frozen Visium, as well as FFPE Visium, Visium setup with CytAssist, and the latest Visium system, Visium HD! See more here! Each Visium Spatial Gene Expression Slide (Fresh Frozen) includes 4 capture areas (6.5 x 6.5 mm) each defined by a fiducial frame.

The Visium platform from 10x Genomics combines histology and spatially resolved whole transcriptome gene expression profiling to localize and quantify gene expression in the tissue context. The UMGC offers two assays for spatial analysis: Visium HD at single cell-scale resolution; Spatial Gene Expression (Visium Standard) at 55 uM resolution

Answer: For a Visium HD capture area fully covered by tissue (100%), the sequencing recommendation is a minimum of 275 million read pairs. Sequencing is highly tissue block and tissue type dependent. For RNA-rich samples, more information can be accessed by increasing sequencing reads, in some cases by 2x or more.

2025年1月9日 · Learn how 10X Visium Spatial Transcriptomics is revolutionizing research in oncology, neuroscience, and biomarker discovery with high-resolution insights

2024年3月14日 · Visium offers a range of sample handling and library construction methods which introduces a need for benchmarking to compare data quality and assess how well the technology can recover expected tissue features and biological signatures.