MegAlign Pro offers a complete sequence alignment software package, including everything you need for each stage of an alignment – not only the algorithms needed for aligning both gene-level and genome-scale sequence data, but also the capability to dig deep in the post-alignment stage.

MegAlign Pro is Lasergene’s application for generating multiple and pairwise alignments of protein and/or nucleic acid sequences using your choice of alignment methods. Easily navigate between synchronized views with a click of a button, and rearrange views and toolbars by simply dragging and dropping them where you want them to be.

Align by ClustalW:用于比对任意数量的同源核苷酸或蛋白质序列。 使用渐进式比对方法。 在这些序列中，最相似的序列，即那些具有最佳比对分数的序列首先被比对。 然后逐渐对更远的序列组进行比对，直到获得全局比对， 不同序列间的位点你可以选择同一列的，也可以是错开的,要根据自己的目的进行选择。 PAIRWISE ALIGNMENT parameters control the speed-sensitivity of the …

Perform accurate multiple sequence alignments of DNA, RNA, and protein sequences for both gene-level and genome-scale sequence data, then analyze in-depth. Use MegAlign Pro for accurate sequence alignment and in-depth analysis.

DNAstar软件共有七个小程序（见下图），各自执行不同的功能，Editseq用于序列编辑，Seqman可以去除载体序列和拼接序列，MegAlign则主要执行序列比对的功能．

MEGA is an integrated tool for conducting automatic and manual sequence alignment, inferring phylogenetic trees, mining web-based databases, estimating rates of molecular evolution, and testing evolutionary hypotheses.

MegAlign Pro 可以快速轻松地执行多重和成对序列比对，然后指导您完成比对后过程，让您生成系统发育树、评估变体、分离有趣的区域以进 行新的子比对、编辑和修剪单个序列或整个比对，并在生成适合发布的高质量图像之前自定义比对的外观。

MegAlign Pro offers a complete sequence alignment software package, including everything you need for each stage of an alignment – not only the algorithms needed for aligning both gene-level and genome-scale sequence data, but also the capability to dig deep in the post-alignment stage.

MegAlign Pro丰富的图形界面支持您加载各种分类群(序列)，执行多序列比对，然后构建高质量的系统发育树; 这一系列操作通常从开始到结束只需几分钟。并且他们都包含在一个应用程序中。 这篇文章的Part A，我将讲述如何使用MegAlign Pro来创建一个系统发育树。

MegAlign Pro 可指导您完成比对后过程，包括生成和比较多个系统发育树，以及识别和分析基因组菌株中的变异。您可以轻松地为新的子比对分离感兴趣的区域，编辑和修剪单个序列或整个比对，并在生成适合发布的高质量图像之前自定义比对外观。