2021年1月8日 · In this study, we established a hyperplastic intestinal organoid culturing system, which shared regenerative features with hyperplastic intestinal epithelium in vivo.

Human intestinal organoids derived from adult stem cells are miniature ex vivo versions of the human intestinal epithelium. Intestinal organoids are useful tools for the study of intestinal physiology as well as many disease conditions.

2024年12月23日 · Currently, there are several approaches and methodologies to establish intestinal organoid co-cultures, and each approach has its own strengths and limitations.

2018年6月25日 · We provide detailed protocols for characterizing the coculture with regard to bacterial and organoid cell viability and growth kinetics. Spatial relationships can be studied by fluorescence...

Intestinal organoids can be generated from adult stem cells and pluripotent stem cells. Organoid culture system excels at modeling disease and patient response, as well as expanding intestinal stem cells indefinitely. PSC-derived intestinal organoids are capable of generating highly complex intestinal tissue including submucosal to mucosal layers.

Since the development of the three-dimensional (3D) “mini-gut” culture system, adult stem cell-derived organoid technology has rapidly advanced, providing in vitro models that replicate key cellular, molecular, and physiological properties of multiple organs.

Intestinal organoid cultures are a powerful tool to study epithelial cells in vitro, as they are able to proliferate and differentiate into all cell lineages observed in vivo.

2025年2月19日 · 2.2 Biomaterials for Intestinal Organoid Culture. The media utilised for organoid culture primarily include Matrigel, a natural medium obtained from Engelbreth-Holm-Swarm mouse sarcoma cells , which is widely considered the “gold standard” scaffold for in vitro cell growth and the first-generation extracellular matrix (ECM) for organoid ...

Here, we established a novel intestinal organoid culture system composed of 8 components, mainly including VPA, EPZ6438, LDN193189, and R-Spondin 1 conditioned medium, which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury; therefore, these organoids were designated hyperplastic intestinal organoids (Hype...

The development of sustainable intestinal organoid cell culture has emerged as a new modality for the study of intestinal function and cellular processes. Organoid culture is providing a new test-bed for therapeutic research and development.