Explore Bio-Rad’s comprehensive pGLO and GFP teaching resources, including pGLO plasmid maps, hands-on lab activities, and free presentations, videos, and case studies. Learn how to use GFP to illuminate gene regulation and expression in your classroom.

The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes , most notably the green fluorescent protein (GFP) and the ampicillin resistance gene.

pGLO. How is the pGLO plasmid introduced into the E. coli cell? Expression of GFP; Genetic transformation is the process by which an organism acquires and expresses a new gene. Genetic engineering is the directed transfer of a gene, or piece of DNA, into a cell (typically a bacteria).

Bacterial transformation plasmid for regulated expression of GFP. Sequence Author: Bio-Rad. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. Efficient cleavage requires at least two copies of the SgrAI recognition sequence. Sticky ends from different BsmBI sites may not be compatible.

Bio-Rad's pGLO Bacterial Transformation Kit is the classic kit for teaching the central dogma and the basics of genetic engineering. In this bacterial transformation lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria , which causes the ...

2019年1月1日 · Here, I describe some extensions of the pGLO transformation experiment that can be used to explore aspects of the system in more detail and to demonstrate other biological concepts. A key feature of the pGLO system is the interaction between the sugar L-arabinose and the AraC protein.

What is Transformation? What is a plasmid? Reasons for Performing Each Transformation Step? Why Perform Each Transformation Step? 2. Incubate on ice. 3. Heat-shock. 4. Nutrient broth …

Cells which were treated with DNA (+pGLO) should contain the pGLO plasmid and should express the ampicillin resistance gene—the corresponding LB/amp plate will contain transformed bacterial colonies.