wpre是一段顺式作用的rna元件，为转录后调控序列，放在多聚腺苷酸化信号之前可显著增加mrna的表达水平和翻译效率，进而增强基因的表达，并且wpre插入病毒载体中还可以大大提升病毒包装的滴度。

1.WPRE不是polya的作用，是能够提高病毒产量的，只在病毒载体才会用。病毒载体是用3LTR充当. polya的作用，中间不能有polya会终止病毒完整RNA转录

Woodchuck Hepatitis Virus (WHV) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed, creates a tertiary structure enhancing expression. The sequence is commonly used in molecular biology to increase expression of genes delivered by viral vectors. [1]

2007年6月28日 · WPRE, when placed upstream of the RV LTRs, significantly reduced readthrough transcription. Readthrough, present at much lower levels with the SIN HIV-1 LV-LTR, was also reduced with WPRE.

2015年10月21日 · Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of …

The expression of genes delivered by retroviral vectors is often inefficient, a potential obstacle for their widespread use in human gene therapy. Here, we explored the possibility that the posttranscriptional regulatory element of woodchuck hepatitis …

The woodchuck hepatitis virus has a post-transcriptional regulatory element (WPRE) and our investigators found that it can substantially stimulate the expression of transgenes delivered by retroviral vectors, including spliced mRNAs and intronless transcripts.

目前多数慢病毒载体的另一个元件是肝炎病毒土拨鼠转录后调控元件(WPRE) ，该元件如果置于转基因序列的正义方向下游，能使表达效率提高5~8 倍( Zufferey et al. 1999) 。

2005年12月15日 · Schematic design of WPRE elements and vectors. ( a ) The post-transcriptional regulatory element (PRE) from Woodchuck Hepatitis Virus B (WHV) is drawn as a gray bar; nucleotide numbering is ...

The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) increases transgene expression from a variety of viral vectors, although the precise mechanism is not known. WPRE is most effective when placed downstream of the transgene, proximal to the polyadenylation signal.