2024年9月27日 · WGBS is a method to detect the DNA methylation status of nearly all the cytosines in the input sample. Genomic DNA is bisulfite converted, the library is prepared through a single- or double-stranded library preparation approach, and sequencing is done to generate single-base resolution data.

2019年7月17日 · Whole genome bisulfite sequencing (WGBS), with its ability to interrogate methylation status at single CpG site resolution epigenome-wide, is a powerful technique for use in...

Whole Genome Bisulfite Sequencing is used to investigate DNA methylation patterns to base granularity. A bisulfite treatment converts cytosines into uracils, but leaves methylated cytosines unchanged.

2018年3月15日 · Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available …

Whole-genome bisulfite sequencing (WGBS) is a high-throughput method for analyzing DNA methylation. This technique involves library preparation, alignment, and quality control. Advancements in epigenetic technology have led to an increase in DNA methylation studies.

2024年6月24日 · NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data.

2022年6月27日 · NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data.

2013年9月26日 · Here we describe a detailed protocol for tagmentation-based WGBS (T-WGBS) and demonstrate its reliability in comparison with conventional WGBS. In T-WGBS, a hyperactive Tn 5 transposase...

Although bulk whole genome bisulfite sequencing (WGBS) has made great efforts in mapping the DNA methylome landscape across types of tissues, it still has certain deficiencies in explaining the cell heterogeneity and understanding the development dynamics in specific biological status.

The study of cytosine modifications is central to the understanding of how transcriptional regulation is maintained in a cell type, as DNA methylation (5-methylcytosine, 5mC) can be propagated through mitosis to daughter chromatids (Jeltsch 2006) and can therefore mediate the “persistent homeostasis” described by Nanney as the defining characteristic of cellular …