2006年3月24日 · RAD53 encodes an essential protein kinase required for cell cycle checkpoint function. By modifying the phosphorylation state of protein targets, Rad53p amplifies initial signals from proteins that recognize DNA damage and replication blocks (11, 14, and reviewed in 16).

2020年8月19日 · Rad53-Spt21 mutants display severe glucose dependence, caused by excess histones through two separable mechanisms: dampening of acetyl-coenzyme A-dependent carbon metabolism through histone...

2001年1月1日 · The evolutionarily conserved yeast checkpoint protein kinase Rad53 regulates cell cycle progression, transcription, and DNA repair in response to DNA damage. To uncover potential regulatory targets of Rad53, we identified proteins physically associated with it in vivo using protein affinity purification and tandem mass spectrometry.

2003年11月26日 · Cells lacking Rad53 are hypersensitive to histone overexpression and fail to degrade excess histones. Taken together, these data establish that Rad53 exerts a control on histone stability. Interestingly, both survival to histone overexpression and the ability to degrade histone require the intact kinase domain of Rad53 and are independent of Mec1.

2020年4月27日 · In vitro assays using a fully reconstituted DNA replication system reveal that the checkpoint kinase Rad53 restrains CMG helicase activity to prevent DNA unwinding and collapse of stalled forks...

2021年8月13日 · The Rad53 DNA checkpoint protein kinase plays multiple roles in the budding yeast cell response to DNA replication stress. Key amongst these is its enigmatic role in safeguarding DNA replication forks. Using DNA replication reactions reconstituted with purified proteins, we show Rad53 phosphorylatio …

2004年9月17日 · Rad53, the Saccharomyces cerevisiae homolog of Chk2, is essential for the checkpoint response to DNA damage and replication block signals as well as normal cell growth (reviewed in Ref. 10).

2006年8月15日 · In budding yeast, the serine/threonine kinase Rad53 (Chk2) is essential for checkpoint control. Rad53 becomes phosphorylated in response to both DNA damage and incomplete replication, in a manner that is dependent on sensor proteins, and on Mec1 (Fig. 1).

2025年2月9日 · Rad53 prevents the generation of excessive ssDNA under replication stress by coordinating DNA unwinding with synthesis of both strands. Rad53 can display active conformations and phosphor-priming confers functionally relevant autophosphorylation.

2005年9月20日 · Recent studies have elucidated the activation mechanism of the Rad53 checkpoint kinase and the role of Rad9-like adaptor proteins in mediating signal transduction from PIKK sensor kinases that detect DNA damage to the effector kinases that play a …