The carboxy-terminal domain of RNA polymerase II typically consists of up to 52 repeats of the sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser. [32] The domain stretches from the core of the RNAPII enzyme to the exit channel, this placement is effective due to its inductions of "RNA processing reactions, through direct or indirect interactions with ...

釀酒酵母的RNA聚合酶Ⅱ包含有12个亚基 [4] 。. 早期的研究表明至少有两种RNAP：一种在核仁中合成rRNA，另一种在核质中合成其他RNA，核一部分但在核仁外 [5] 。 在1969年，科学实验家罗伯特·罗德（Robert Roeder）和威廉·鲁特（William Rutter）最终发现了一种额外的RNAP，它负责在核质中转录某种RNA。

RNAPII can exist in two forms: RNAPII0, with a highly phosphorylated CTD, and RNAPIIA, with a nonphosphorylated CTD. [16] Phosphorylation occurs principally on Ser2 and Ser5 of the repeats, although these positions are not equivalent.

RNA聚合酶Ⅱ（英语：RNA polymerase II，亦被称为RNAPⅡ或PolⅡ）是一种存在于真核生物细胞中的酶。它催化脱氧核糖核酸转录，合成mRNA及大多数hnRNA和微RNA的前体。

RNA polymerase II (Pol II) is a multi-subunit enzyme involved in transcribing all protein-coding genes in eukaryotes. 1,2 The largest subunit of Pol II (RPB1) contains a series of seven residue (Heptad) repeats as its Carboxyl-Terminal domain (CTD). The CTD interacts with various general and specific transcription factors, regulators, and other proteins to aid and regulate …

2025年2月6日 · Our results suggest that the dynamic regulation of RNA-DNA hybrid formation at stalled forks by RNAPII and DDX39A precisely governs the timing of DNA2 activation, contributing to stalled fork protection, processing, and restart, ultimately promoting genome stability.

2023年8月24日 · We show the ATPase WRNIP1 associates with RNAPII and limits ATM activation during unperturbed cell cycle. WRNIP1 binding to elongating RNAPII requires catalytic activity of the ubiquitin ligase...

通过防止RNA聚合酶II (RNA polymerase II，RNAPII)在DNA损伤处的长期停滞，TC-NER确保了转录的迅速恢复和避免细胞凋亡。TC-NER是由DNA损伤处拉长的RNAPII分子(RNAPII molecules，RNAPIIo)的停滞触发的。

2021年10月22日 · Here, we tested the role of RNA polymerase II (RNAPII) in this transition using a cell line that allows its auxin-mediated degradation. In situ Hi-C showed that RNAPII is required for both compartment and loop establishment following mitosis. RNAPs often counteract loop extrusion, and in their absence, longer and more prominent loops arose.

2021年6月20日 · This review summarizes nearly five decades of work on transcription initiation by describing the sequential recruitment of diverse molecular players including the GTFs, the Mediator complex, and DNA repair factors that support RNAPII to enable RNA synthesis.