Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of …

Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and unknown …

Rapid Amplification of cDNA Ends (RACE) is a procedure for amplification of nucleic acid sequences from a messenger RNA template between a defined internal site and either the 3´ …

2006年12月29日 · RACE (rapid amplification of cDNA ends) PCR is useful for quickly obtaining full length cDNAs for mRNAs for which only part of the sequence is known and to identify …

Rapid amplification of cDNA 5′ ends (5′-RACE) is routinely used for the sequence analysis of the upstream noncoding regions of cellular mRNAs; however, it represents a tedious and cost …

2007年1月11日 · In 3′ RACE, the poly (A) tail functions as a non-specific tag at the 3′ end of the mRNA. cDNA ends can be obtained in 1–3 days using this protocol. PCR can be used to …

We provide a detailed protocol of our 3’ RACE procedure comprising four major parts: Total RNA extraction, RNA amount determination and quality control, the RACE procedure itself, and …

Rapid amplification of cDNA ends (RACE) is a technique used to obtain the full-length sequences of RNA transcripts. RACE can provide the sequence of an RNA transcript from a short, known …

2005年12月1日 · Rapid amplification of cDNA ends (RACE) is a powerful PCR-based technique for determination of RNA terminal sequences. However, most of the RACE methods reported …

1 Overview of the 5' RACE System Procedure..... 3 2 Sequences of the 5' RACE Primers ..... 4 3 Effect of GSP1 on 5' RACE Efficiency ..... 7