pYES2 Sequence and Map - SnapGene
Download pYES2.dna file. Download Plasmid Open in SnapGene. SnapGene. SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. Fast accurate construct design for all major molecular cloning techniques; Validate sequenced constructs using powerful alignment tools;
pYES2 Yeast Expression Vector - Thermo Fisher Scientific
The pYES2 vector is designed for native expression of your protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2 origin for high-copy maintenance.
pYES2 NT C Sequence and Map - SnapGene
High-copy episomal vector for galactose-inducible expression of N-terminally tagged proteins in S. cerevisiae. For other reading frames, use pYES2/NT A or pYES2/NT B.
in an expression plasmid (i.e. pYES2/NT), the presence of the 2μ origin allows the plasmid to be episomally maintained and replicated at high copy number (generally 10–40 copies per cell).
1.将目的蛋白克隆到pYES2载体（多克隆位点请参照图谱序列）。 2.将重组载体转化到大肠杆菌感受态细胞中（参照TOP10F’感受态细胞使用说明书）。 3.用含氨苄（50-100ug/mL）的LB平板筛选转化产物。 4.挑选10-20个克隆，PCR并测序验证，筛选阳性克隆。
The parental pYES2 vector (Figure 3) is utilized for native expression of your protein of interest. It contains the URA3 selection marker for selection in yeast. The pYES2/NT vector (Figure 4), derived from the parental backbone of pYES2, offers an N-terminal tag. It contains the Xpress™ epitope for simple detection with Invitrogen’s Anti-
Introduction pYES2 is a 5.9 kb vector designed for inducible expression of recombinant proteins in Saccharomyces cerevisiae . Features of the vector allow easy cloning of
pYES2 载体图谱和序列 - 纽普生物
The pYES2 vector is an episomal high-copy plasmid designed for galactose-inducible protein expression in Saccharomyces cerevisiae.
pYES2质粒图谱、载体图谱、序列、价格、抗性、测序引物、大小 …
pYES2的是一个5.9 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。 载体的特点在于基因插入载体的构建简单，以及能够使用原养型尿嘧啶进行转化株的筛选。
pYES2/CT, pYES3/CT, and pYC2/CT are 6.0 kb, 5.9 kb, and 4.6 kb vectors, respectively, designed for inducible expression of recombinant proteins in Saccharomyces cerevisiae. Features of the vectors allow purification and detection of expressed proteins (see pages 13–20 for more information). The vectors contain the following elements: