PTA’s ability to successfully stain the brain and permit specific layer identifications was recognized early by Metscher (2009a) and has further proven to provide superior contrast …

contrast agent, phosphotungstic acid (PTA) is used to stain mouse embryos for micro-CT scanning. PTA has the chemical formula H 3 PW 12 O 40 (see the sidebar from the Wikipedia …

When utilizing I2KI, PMA, and PTA stains, 1% PMA staining for 24 h at both 25 °C and 40 °C resulted in the biggest visualized embryo volume relative to no stain, whereas 3% I2KI staining ...

2014年4月25日 · This study describes a perfusion approach using iodine or phosphotungstic acid (PTA) stains, delivered to an intact mouse, to capitalize on the microvasculature as a delivery …

The presented method is compatible with standard enzymatic in situ hybridization protocols and applicable both to unstained specimens and soft-tissue samples stained with phoshotungstic …

(1 part PMA, 1 part PTA, 2 parts MilliQ water): Now referred to as PMA/PTA Solution. Place slides in the Fume Hood in a container of Bouin’s Solution, either overnight at Room Temperature …

We provide detailed protocols for micro-level visualization of soft tissue structures in mice at several embryonic and early postnatal ages using PTA-enhanced microCT.

2017年2月8日 · The aim of the study was to establish a phosphotungstic acid (PTA) ex-vivo staining approach that efficiently enhances the x-ray attenuation of soft-tissue to allow high …

The aim of the study was to establish a phosphotungstic acid (PTA) ex-vivo staining approach that efficiently enhances the x-ray attenuation of soft-tissue to allow high resolution 3D visualization …

2019年11月18日 · We provide detailed protocols for micro-level visualization of soft tissue structures in mice at several embryonic and early postnatal ages using PTA-enhanced microCT.