PE-Cyanine®5 (PE-Cy5) is a red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy5. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy5.

PE-Cy5 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by the 488 nm light of an Argon ion laser and serves as an energy donor, coupled to the cyanine dye Cy5, which acts as an energy acceptor and fluoresces at 670 nm.

2017年6月2日 · Absorption and emission spectra of FITC (Green), PE-Cy5 (Orange) and PE-Cy7 (Red). All three fluorophores can be well excited by the 488 nm laser line (Blue) with excellent spectral seperation.

2025年2月28日 · PE-Cy5.5 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at~700 nm. The filter sets of 682/33 nm and 695/40 nm are recommended for this tandem color.

Cyanine5 (Cy5) is a bright, far-red-fluorescent dye with excitation suited for the 633 nm or 647 nm laser lines and is used for imaging, flow cytometry and genomics.

DRAQ5TM (ab108410) allows rapid staining of dsDNA/nuclei of LIVE or FIXED cells. It is ideal for use in multi-color analysis and compatible with GFP labels. As well as offering half the scan times of Hoechst or DAPI, DRAQ5TM can be used in a greater variety of applications.

2025年3月4日 · PE-Cy5 is a popular color used in flow cytometry. Our preactivated PE-Cy5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE-Cy5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets ...

PE-Cy5是由PE和Cy5这两种荧光素通过共价结合在一起。 PE-Cy5是 流式细胞术 中常用的颜色，荧光素在受到适当波长的光线照射时，会释放出波长比照射光长的光，这个现象叫做荧光。 激发光和释放光波长的差异是每种荧光素的分子特性所决定的。 PE的最佳激发光在560纳米，而最强释放光在570纳米左右。 而合成PE-Cy5后，由于两个分子的距离很小，释放光还没有离开，就 …

PE-Cy5 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by the 488 nm light of an Argon ion laser and serves as an energy donor, coupled to the cyanine dye Cy5, which acts as an energy acceptor and fluoresces at 670 nm.

2020年11月19日 · PE-Cy5, PE-Cy5.5, PerCP-Cy5.5, PE-Dazzle 594, BV605, and BV650 are some examples of what I call “high perpetrators”, meaning that these fluorophores spill into a lot of other detectors or have a large amount of spill into another detector.