mRuby3 is a basic (constitutively fluorescent) red fluorescent protein published in 2016, derived from Entacmaea quadricolor. It has low acid sensitivity.

Sting-/- MEFs被mRuby3-STING和mNeonGreen-泛素稳定地转导，并用Airyscan超分辨率显微镜进行成像。 与EGFP-STING8一样，mRuby3-STING在DMXAA刺激后30分钟转移到核周围的高尔基体，然后在120分钟之前转移到REs。

2016年2月16日 · Here we report the engineering of brighter and more photostable variants, mClover3 and mRuby3. mClover3 improves photostability by 60% and mRuby3 by 200% over the previous generation of...

Monomeric red fluorescent protein derived from mRuby2, with improved brightness and photostability. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More …

Plasmid pNCS-mRuby3 from Dr. Michael Lin's lab contains the insert mRuby3 and is published in Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. This plasmid is available through Addgene.

2020年2月1日 · Here we established the fluorescent protein mRuby3 as tag for efficient in vivo protein localization studies by expressing a codon-optimized gene in P. tricornutum. mRuby3 was directed to seven different subcellular localizations by means of full-length marker protein or N-/C-terminal targeting signal fusions; its emission was detected ...

2021年3月26日 · 在光漂白动力学方面，mruby3表现出单指数关系，它的解离常数值为4.8，与mruby2相比耐酸性相似。 mruby3是目前为止亮度比较亮和光稳定好的红色荧光蛋白。

Optimized mRuby3 is a Suitable Fluorescent Protein for in vivo Co-localization Studies with GFP in the Diatom Phaeodactylum tricornutum. Phaeodactylum tricornutum is an ecologically and evolutionarily relevant microalga that has developed into an important model for molecular biological studies on organisms with complex plastids.

2009年2月5日 · With excitation and emission maxima at 558 nm and 605 nm, respectively, and a large Stokes shift of 47 nm, mRuby appears particularly useful for imaging applications. The protein shows an exceptional resistance to denaturation at pH extremes.

2016年2月16日 · Here we report the engineering of brighter and more photostable variants, mClover3 and mRuby3. mClover3 improves photostability by 60% and mRuby3 by 200% over the previous generation of fluorophores. Notably, mRuby3 is also 35% brighter than mRuby2, making it both the brightest and most photostable monomeric red FP yet characterized.