The herpes simplex virus type 1 (HSV-1)-encoded thymidine kinase (TK) appears to be such a protein. HSV-1 TK is a multifunctional enzyme that possesses kinase activities normally performed by three separate enzymes.

22 小时之前 · To deplete male germ cells using HSV-TK, we inserted the P2A self-cleaving peptide and HSV-thymidine kinase (HSV-TK) gene into the C-terminus of the germ cell-specific gene, Vasa (a.k.a. Ddx4 or ...

Herpes Simplex Virus type 1 (HSV-1) thymidine kinase (TK) is currently the most widely used suicide agent for gene therapy of cancer. Tumor cells that express HSV-1 thymidine kinase are rendered sensitive to prodrugs due to preferential phosphorylation by this enzyme.

2022年9月8日 · SHED-TK demonstrated a strong bystander effect in vitro and in vivo and completely eradicated H1299 NSCLC in the brain. SHED-TK cells implanted intratumorally followed by GCV administration significantly suppressed the growth of …

2003年3月24日 · Here, we demonstrate that Epstein-Barr virus (EBV)–specific T cells transduced with vectors encoding herpes simplex virus-1 thymidine kinase (HSV-TK) selectively accumulate radiolabeled...

HSV resistance to ACV has been associated with mutations occurring either in the viral TK gene or the viral DNA polymerase gene. Three mechanisms of resistance have been proposed: a loss of TK activity (TK-deficient virus), an alteration of TK substrate specificity (TK-altered virus), and/or an alteration of DNA polymerase activity (15).

2006年2月3日 · Here a new optimized derivative of the herpes simplex virus 1-thymidine-kinase (HSV1-TK) gene is described. The HSV-TK gene is frequently used in experimental studies on gene-directed...

1995年6月12日 · The herpes simplex virus thymidine kinase (HSV-TK) converts ganciclovir (GCV) into a toxic product and allows selective elimination of TK+ cells in vitro and in vivo. It is currently being used in clinical gene therapy trials as a therapeutic gene or as a safety marker.

Herpes simplex virus (HSV) thymidine kinase (TK) expression and the HSV TK gene have been evaluated in studies of gene control, as well as in animal and human studies of viral pathogenesis, including HSV latency.

Herpes simplex virus type 1 (HSV 1) thymidine kinase (TK) exhibits an extensive substrate diversity for nucleobases and sugar moieties, in contrast to other TKs. This substrate diversity is the crucial molecular basis of selective antiviral and suicide gene therapy.