The GFP-Trap Magnetic Particles M-270 Kit contains GFP-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of GFP-fusion proteins and their interacting factors. • IP and Co-IP of GFP-tagged proteins without size limitation

The ChromoTek GFP-Trap is the gold standard for fast, reliable and effective one-step immunoprecipitation of GFP-fusion proteins and their interacting factors from cell extracts or organelles. Dissociation constant K of 1pM. Fulfi lls highest requirements for antibody validation. Structure and function are characterized.

The GFP trap utilizes the distinctive fluorescent properties of green fluorescent protein (GFP) to tag proteins of interest, enabling researchers to visualize interactions and localization in real time.

2022年9月16日 · In this chapter, we describe a method to co-immunoprecipitate the fluorescently tagged candidate protein with its interacting partners from the crude plant cell extracts using green fluorescent protein (GFP)-trap magnetic beads.

2022年2月14日 · GFP traps (Morphotrap and the GrabFP system) are based on tissue-specific expression of an anti-GFP nanobody (vhhGFP4) fused to different transmembrane domains targeted to distinct cell compartments.

Here, we describe the implementation of magnetic beads and high-affinity anti-green fluorescent protein (GFP) antibodies to develop an in vitro GFP-protein trap-like system.

2023年7月15日 · Here, we describe the implementation of magnetic beads and high-affinity anti-green fluorescent protein (GFP) antibodies to develop an in vitro GFP-protein trap-like system.

Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis