Escherichia coli possesses three distinct formate dehydrogenase enzymes encoded by the fdnGHI, fdhF, and fdoGHI operons. To examine how two of the formate dehyrogenase operons (fdnGHI and fdhF) are expressed anaerobically in the presence of low, ...

2014年8月25日 · In this work, genetic tools are reported that allow the facile isolation of FHL in a single chromatographic step. The core complex is shown to comprise HycE (a [NiFe] hydrogenase component termed Hyd-3), FdhF (the molybdenum-dependent formate dehydrogenase-H), and three iron-sulfur proteins: HycB, HycF, and HycG.

2022年9月14日 · We evaluate possible structural determinants of the bias toward hydrogen production over its oxidation and describe an unpredicted metal-binding site near the interface of FdhF and HycF...

We evaluate possible structural determinants of the bias toward hydrogen production over its oxidation and describe an unpredicted metal-binding site near the interface of FdhF and HycF subunits that may play a role in redox-dependent regulation of FdhF interaction with the complex.

The formate dehydrogenase subunit FdhF, which shows partial occu- pancy in the aerobically puri fied complex, was entirely absent in the anaerobically isolated complex in multiple replicates of ...

2008年2月26日 · fdhF. Status. UniProtKB reviewed (Swiss-Prot) Organism. Escherichia coli (strain K12) Amino acids. 715 (go to sequence) Protein existence. Evidence at protein level. Annotation score. 5/5. Entry. Variant viewer. Feature viewer. Genomic coordinates. Publications. External links. History. Tools.

The structural gene (fdhF) for the 80-kDa selenopolypeptide of formate dehydrogenase (formate:benzyl viologen oxidoreductase, EC 1.2.--.--) from Escherichia coli contains an in-frame UGA codon at amino acid position 140 that is translated.

In striking contrast to many other anaerobic-specific genes, which require DNA to be negatively supercoiled for expression, we have found that inhibition of DNA gyrase activity enhances expression from the gene (fdhF) encoding the selenopolypeptide of FDHH.

The direction of transcription of gene fdhF was found to be counterclockwise on the E. coli chromosome in one Mudl(Aprlac) fusion mutant. Expression of the lac operon in this mutant was induced by formate and repressed by nitrate, nitrite or trimethylamine N-oxide.

2024年10月9日 · It is known that both the molybdenum-containing E. coli FdhF formate dehydrogenase (a component of the organism’s fully reversible formate:hydrogen lyase complex) and the molybdenum-containing Cupriavidus necator FdsDABG formate dehydrogenase catalyze the transfer of the C α hydrogen of substrate to their metal centers in the course of the ...