The Expi293 Expression System provides rapid, high-yield protein production in mammalian cells by combining high-density Expi293F cells, an optimized medium, specialized transfection reagent and enhancer.

The GibcoTM Expi293TM Expression System is a high-yield transient expression system based on suspension-adapted Human Embryonic Kidney (HEK) cells. The Expi293TM Expression System Kit provides cells, culture medium, and reagents to transfect a …

Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium.

The Expi293 Expression System is a complete solution for rapid, high-yield protein production from mammalian 293 cells. It is based on integrated components including high-density Expi293F cells in Expi293 Expression Medium and the cationic lipid-based ExpiFectamine 293 transfection reagent in combination with specialized transfection enhancers.

Expi293F™ Cells are human cells derived from the 293F cell line, and are a core component of the Expi293™ Expression System. They They are maintained in suspension culture and optimized to grow to high density in Expi293 ™ Expression Medium.

Each Expi293TM Expression System Starter Kit provides cells, culture medium, and reagents to express a total of 1 liter production volume. The structural biology and inducible expression modules of the Expi293TM Expression System includes four Expi293FTM cell lines that can be used to express your protein of interest.

Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of HEK 293 cells. The Expi293™ Expression Medium is formulated with GlutaMAX™ I Supplement.

The Expi293™ Expression System facilitates large-scale transfection of suspension 293 human embryonic kidney cells in a defined, serum-free medium for expression of proteins and virus. ∤ Transfection and expression experiments may be performed directly in Expi293™ Expression Medium without the need for media change. ∤

Here, we describe a protocol that can produce functional tetravalent, bispecific antibodies at around 22 mg protein/l to a low cost. The expression system is based on the Expi293 cells, which have been adapted to grow in denser cultures than …

The Expi293 Expression system is a rapid, scalable system that delivers the highest protein yields of any current transient expression method. Typical Expi293 yields are 2- to 10-fold higher than other options.

The Expi293 and ExpiCHO transient expression systems offer all-in-one solutions for generating high-titer recombinant proteins for a broad range of research applications including candidate drug identification, reagent production, structural biology and …

2022年1月28日 · Transient gene expression (TGE) is an important tool for generating recombinant proteins in a short period of time. The human cell line HEK293 is widely used for this purpose since it can grow in suspension to a high cell density in serum-free media.

Each Expi293™ Expression System Kit contains one of the following four Expi293F™ cell lines for expression of proteins of interest. • Use Expi293F ™ cells for routine protein expression tasks.

Gibco Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein ...

provides users with an enriched sample of functional GPCRs and other cell-surface membrane proteins. This is accomplished through a less labor-intensive and easier workflow than used to prepare plasma mem.

2017年9月15日 · Methods: Here, we describe a protocol that can produce functional tetravalent, bispecific antibodies at around 22 mg protein/l to a low cost. The expression system is based on the Expi293 cells, which have been adapted to grow in denser cultures than HEK293 cells and gives higher expression yields.

The Expi293FTM cell line is a variant of the 293 cell line, which is adapted to high-density suspension growth in Expi293TM Expression Medium.

MembraneProTM Reagent: This reagent is optimized for high-yield packaging and secretion of virus-like particles (VLPs) that contain the functional membrane protein of interest into the extracellular medium.

2025年3月12日 · In further work, we found that HARS WHEP-Fc bound with a median effective concentration (EC 50) around 30 nM to NRP2 overexpressed on HEK Expi293 cells . A control protein NH-Fc (IgG1-Fc fused with only amino acids 2 to 11 of HARS WHEP and missing amino acids 12 to 60) showed little binding to these cells.

The Expi293 Expression System provides rapid, high-yield protein production in mammalian cells by combining high-density Expi293F cells, an optimized medium, specialized transfection reagent and enhancer.