DsRed surprisingly takes days at room temperature to reach full red fluorescence. At room temperature, a sample of purified protein initially shows a major component of green fluorescence (ex/em 475/499 nm), which peaks in intensity around 7 h and decreases to nearly zero over 2 days. Meanwhile the red fluorescence reaches half its maximal ...

Red fluorescent protein (RFP) is a protein which acts as a fluorophore, fluorescing red-orange when excited.The original variant occurs naturally in the coral genus Discosoma, and is named DsRed.Several new variants have been developed using directed mutagenesis [1] which fluoresce orange, red, and far-red. [2]

2002年1月1日 · The red fluorescent protein DsRed has spectral properties that are ideal for dual-color experiments with green fluorescent protein (GFP)1. But wild-type DsRed has several drawbacks, including slow ...

Based on the pUG plasmids, a new set of vectors expressing DsRed was constructed, allowing the production of amino-terminal (N-terminal) and carboxyl-terminal (C-terminal) fusion proteins. Our results indicate that DsRed can be expressed in S. cerevisiae, and the protein can be targeted specifically to the nucleus. Finally, we show that cells ...

A DsRed derivative, known as mNectarine, exhibits a strong pH-dependent fluorescence in physiological pH range with apparent pKa value about 6.9 (Johnson et al., 2009). The mNectarine fusion with human concentrative nucleoside transporter was used to measure pH during the H + –nucleoside cotransport in live cells. However, the calibration ...

2019年1月3日 · Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed) Bevis Bj, Glick Bs (2002). Nature Biotechnology, 20(1) , 83-87. doi: 10.1038/nbt0102-83. Article Pubmed. Add a reference. External Resources. Search Addgene for DsRed2; Search RIKEN BRC for DsRed2; Change history.

DsRed, a bright red fluorescent protein recently cloned from a corallimorpharian of the Discosoma genus, has considerable potential to complement existing uses of the extremely popular Aequorea victoria green fluorescent protein (avGFP) (for reviews, see refs.1 and 2).Several fluorescent proteins (FPs) homologous to avGFP have been discovered in Anthozoa representatives.

dsRed Express was developed to accelerate chromophore formation. Through key mutations, this variant fluoresces within hours rather than days. One improvement was stabilizing intermediate oxidation states, expediting the transition from the green-emitting precursor to the red fluorescent form. Additionally, dsRed Express exhibits reduced ...

To overcome the slow maturation of DsRed, we used random and directed mutagenesis to create variants that mature 10–15 times faster than the wild-type protein.An asparagine-to-glutamine substitution at position 42 greatly accelerates the maturation of DsRed, but also increases the level of green emission.Additional amino acid substitutions suppress this green emission while …

DsRed is an interesting marker protein for use in plants because of its red-shifted wavelength spectrum that will avoid damaging cells and tissues by excitation light. In this report, we show that DsRed is an excellent marker in higher plants in spite of the interfering red autofluorescence of chlorophyll, which can be eliminated by using the ...