DNMT3 is a family of DNA methyltransferases that could methylate hemimethylated and unmethylated CpG at the same rate. The architecture of DNMT3 enzymes is similar to that of DNMT1, with a regulatory region attached to a catalytic domain.

2023年2月7日 · DNA甲基化是指以共价结合方式获得一个甲基（CH3）基团的过程，其是在DNA序列特定碱基的DNMT催化下作为CH3供体的S-腺苷甲硫氨酸（SAM）的化学修饰过程。 在此过程中，胞嘧啶（C）是甲基化最常见碱基，且C位点甲基化主要发生在CpG序列上。 在哺乳动物中，部分CpG二核苷酸分散在基因组中，而另一些则出现在CpG岛的密集簇中。 CpG岛通常是未甲基化的，常位于调控转录起始和抑制的基因启动子区域中。 增强子或启动子中CpG位点高甲基化通 …

2020年7月3日 · Through comprehensive structural, enzymology and cellular characterization of DNMT3A and DNMT3B, we here report a multi-layered substrate-recognition mechanism underpinning their divergent genomic...

研究显示，DNMT2的功能主要是催化RNA的甲基化，而DNMT3L并不具备催化活性，所以我们在谈论DNMT的时候，一般指的是DNMT1和DNMT3a/3b。 虽然DNMT1和DNMT3a/3b这两个蛋白都具有甲基转移酶的活性，但它们对DNA的修饰方式有所不同。 我们知道，DNA是双链结构，双链DNA的任意一条链上的C都可能被甲基化成5-mC。 在DNA复制的时候，DNA双链打开形成复制叉，然后执行DNA复制功能的蛋白复合物分别以正义链和反义链分别为模板合成新的双链DNA …

2017年10月16日 · DNMT1, DNMT3A and DNMT3B are canonical cytosine-5 DNMTs that catalyse the addition of methylation marks to genomic DNA. By contrast, DNMT2 and DNMT3L are non-canonical family members, as they do...

2022年3月4日 · DNA methylation, one of the best-characterized epigenetic modifications, plays essential roles in development, aging and diseases. The de novo DNA methyltransferase DNMT3A is responsible for the establishment of de novo genomic DNA methylation patterns and, as such, involved in normal development as well as in many diseases including cancer.

2018年2月7日 · Here we report a 2.65-ångström crystal structure of the DNMT3A–DNMT3L–DNA complex in which two DNMT3A monomers simultaneously attack two cytosine–phosphate–guanine (CpG) dinucleotides, with the...

This review focuses on recent developments in the characterization of the DNMT3 family of DNA methyltransferases at the biochemical, structural, and functional levels. Interconnections between the DNA-based and histone-based layers of epigenetic information are particularly highlighted, as it is now clear that de novo methylation occurs chiefly ...

近日，我院蓝斐研究员、吴飞珍副研究员和沈宏杰青年研究员合作在Protein & Cell 在线发表题为“DNMT3A Reads and Connects H3K36me2 to DNA Methylation”的短文，报道了DNMT3A可以通过识别H3K36me2 催化基因间区DNA甲基化建立这一机制，并在多发性骨髓瘤细胞系统中证明这一机制对于肿瘤增殖的潜在生物意义。...

Five DNMT enzymes have been identified in mammals including DNMT1, DNMT2, DNMT3A, DNMT3B, and DNMT3L. DNMTs can be used for the research of epigenetic mechanisms. DNA 甲基转移酶 (DNMT) 参与 DNA 甲基化 DNA methylation，负责将甲基添加到胞嘧啶-磷酸-鸟嘌呤 (CpG) 和非 CpG 二核苷酸位点内的胞嘧啶残基的第五个碳原子上。 已在哺乳动物中鉴定出五种 DNMT 酶，包括 DNMT1、DNMT2、DNMT3A、DNMT3B 和 DNMT3L。 DNMTs 可用于表观 …