The DnaB protein is a major replicative DNA helicase which moves along the lagging strand in the 5′→3′ direction and opens up the duplex DNA at the tip of the replication fork to expose a pair of single-stranded DNA templates. The DnaG protein is a primase which synthesizes a short RNA to prime DNA chain elongation catalyzed by Pol III HE.

2017年4月6日 · To better understand the structure and function of primase and address the interactions between DnaG and specific DNA template, we have carried out structural and functional analysis on DnaG...

DnaA is the specialist initiator protein that recognizes the origin and guides the formation of these nucleoprotein complexes [92–94]. To initiate DNA replication, this complex must be activated and undergo large conformational changes that result in the local unwinding of duplex DNA.

The dnaG gene protein: Summary of steps in E. coli DNA Synthesis. Pol Functions: Pol III Structure and function; Termination of DNA replication; ColE1 Plasmid

DnaG is a bacterial DNA primase and is encoded by the dnaG gene. The enzyme DnaG, and any other DNA primase, synthesizes short strands of RNA known as oligonucleotides during DNA replication. These oligonucleotides are known as primers because they act as a starting point for DNA synthesis.

In this study, we obtained the structure of BsuDnaG RPD domain and addressed the interactions between BsuDnaG and the specific template DNA. We found that the integrity of the ZBD/RPD is essential in ssDNA template binding, and the specific template DNA may bind to the primase in an L …

Compounds that inhibit bacterial DnaG primase have been developed using different approaches. In this paper, we provide an overview of the current literature on DNA primases as novel drug targets and the methods used to find their inhibitors.

Mycobacteria contain genes for several DNA-dependent RNA primases, including dnaG, which encodes an essential replication enzyme that has been proposed as a target for antituberculosis compounds. An in silico analysis revealed that mycobacteria also possess archaeo-eukaryotic superfamily primases (AEPs) of unknown function.

2022年3月3日 · DnaG primase is the specialized DNA-dependent RNA polymerase that synthesizes short oligoribonucleotide polymers called primers. It is required because DNA polymerases lack the ability to initiate chain synthesis but are very efficient at …

DnaG is a primase which synthesizes a short RNA to prime DNA chain elongation catalyzed by pol III HE. From: Encyclopedia of Biological Chemistry (Third Edition), 2021