Cyanine5 (Cy5) is a bright, far-red-fluorescent dye with excitation suited for the 633 nm or 647 nm laser lines and is used for imaging, flow cytometry and genomics.

CY5 is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis.

PE-Cyanine®5 (PE-Cy5) is a red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy5. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy5.

Cy5 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 649 nm and an emission peak at 667 nm. Spectrally its similar to Alexa Fluor™ 647.

Cy5/ Cyanine 5 are popular fluorescent dyes compatible with various detection techniques and standard filters. This class includes far-red fluorescence-emitting dyes with excitation/emission maximum 646/662 nm.

Available non-sulfonated dyes incude Cy3, Cy3.5, Cy5, Cy5.5, Cy7, and Cy7.5. Cy ® stands for 'cyanine', and the first digit identifies the number of carbon atoms between the indolenine groups. Cy2 which is an oxazole derivative rather than indolenin, is an exception from this rule.

2024年9月27日 · Cy5® NHS Ester (GE Healthcare) and Cyanine5 NHS ester are chemically equivalent, far-red fluorescent dyes used for labeling peptides, proteins and oligonucleotides, with an excitation wavelength of 651 nm and an emission wavelength of 670 nm.

Type: Near-infrared (NIR) fluorescent dye molecule commonly used in bioconjugation for applications like microscopy, flow cytometry, and in vivo imaging. High brightness: Offers strong fluorescence intensity for sensitive detection. Convenient conjugate labeling: Can be readily attached to biomolecules like proteins and antibodies.

Cyanine5 is an extremely popular fluorophore. This dye emits in red region. It has an outstanding extinction coefficient, and a good fluorescence quantum yield, and is therefore very bright. It is very photostable; its ability to blink in the presence of thiols makes it a dye of choice for STORM super-resolution microscopy.

The 5-Cy5 modification incorporates a fluorescent Cy™ 5 dye at the 5' terminus of an oligonucleotide. 5' Cy5 modified oligonucleotides can be used in a wide array of applications including dual-labeled fluorogenic probes for real-time PCR.

In summary, Cy5 is a highly effective fluorescent dye for a broad range of applications in biological and medical research, offering distinct advantages in terms of fluorescence properties and compatibility with various detection systems.

Cy5 carboxylic acid, fluorescent dye, Cy5 | C34H42N2O5S | CID 131953009 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

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2021年7月20日 · Cy5 and Cy5.5 are both cyanine dyes with several differences between them. Used in Fluorescence Resonance Energy Transfer (FRET), Fluorescence Polarization (FP), and Time Resolved- Fluorescence Resonance Energy Transfer (TR-FRET).

2022年12月20日 · Cyanine dye is derived from cyanine, which is where its name derives from: Cy2, Cy3, Cy5, and Cy7. All of them can be bonded to nucleic acids, peptides, and proteins by their reaction groups. Cy3 and Cy5 are dyes with high extinction coefficient, which are especially suitable for intracellular sensitive peptide localization experiments and are ...

Removal of cleaved blocking groups from bases. Purification with reverse-phase silica gel column which can effectively remove salts. The purity can meet the needs of most molecular biology experiments. PCR amplification, gene synthesis, DNA sequencing, NGS capture probes, NGS Multiplex PCR oligos.

Multiple antigen peptide application is one potent way to produce high-titer anti-peptide antibodies and synthetic peptide vaccines. This system utilizes the α- and ε-amino groups of lysine to form a backbone to which multiple peptide chains can be attached.

Fluorescent tags or probes are ideal for many imaging and diagnostic applications. Applications for fluorescent peptides range from the study of in vivo biomedical imaging, localization studies, peptide-protein interactions, enzyme activity assays, or development of novel disease models.

Cy5.5 is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis.

Other fluorophores like PE, Alexa Fluor™ 750, Alexa Fluor™ 568, IF488, IF647, Cy5, and APC could also be further evaluated and offered. Antibody Fluorescent Labeling Service Key Features. Minimum amount is only 1 mg. Standard packages scale …