ClpXP is a AAA+ protease that uses the energy of ATP binding and hydrolysis to perform mechanical work during targeted protein degradation within cells. ClpXP consists of hexamers of a AAA+ ATPase (ClpX) and a tetradecameric peptidase (ClpP). Asymmetric ClpX hexamers bind unstructured peptide tags i …

2012年1月1日 · ClpXP is a AAA+ protease that uses the energy of ATP binding and hydrolysis to perform mechanical work during targeted protein degradation within cells. ClpXP consists of hexamers of a AAA+ ATPase (ClpX) and a tetradecameric peptidase (ClpP).

ClpXP is an archetypical and well-studied AAA+ protease present in most eubacteria and the mitochondria of eukaryotic cells (Baker and Sauer, 2012; Olivares et al., 2018). It consists of ClpX, a hexameric AAA+ unfoldase/translocase, and ClpP, a barrel-like peptidase assembled from two heptameric rings.

2019年10月3日 · The ClpXP machinery is a two-component protease complex that performs targeted protein degradation in bacteria and mitochondria. The complex consists of the AAA+ chaperone ClpX and the peptidase...

2020年10月9日 · Mitochondrial ClpP is a stable heptamer ring with peptidase activity that forms a multimeric complex with the ATP-dependent unfoldase ClpX (ClpXP) leading to proteolytic activity. Emerging...

ClpP 是高度保守的 丝氨酸蛋白水解酶，广泛存在原核生物和真核生物中。 十四聚体的ClpP与六聚体的分子伴侣ClpX形成ClpXP复合物，负责水解错误折叠或异常的蛋白。 杨财广组早期针对金黄色葡萄球菌（Sa ClpP）开展调控机制相关的结构和功能研究 [3-5]。 2021年，杨财广组发现ClpP激动剂ONC212可激动水稻白叶枯病菌ClpP（Xoo ClpP），对水稻细菌性叶枯病感染具有较好的救治效果 [6]。 人源ClpP （HsClpP，下统称 ClpP）是线粒体基质中的蛋白水解酶，维持线粒体蛋 …

2021年12月21日 · ClpXP is an archetypical AAA+ protease, consisting of ClpX and ClpP. ClpX is an ATP-dependent protein unfoldase and polypeptide translocase, whereas ClpP is a self-compartmentalized peptidase. ClpXP is currently the only AAA+ protease for which high-resolution structures exist, the molecular basis o …

2018年2月22日 · We improved the activity of ClpXP in cell extract by adding exogenous ATP and an energy regeneration system. We then established conditions for both protein synthesis, and protein degradation by...

2011年4月29日 · We report direct observations of mechanical, force-induced protein unfolding by the ClpX unfoldase from E. coli, alone, and in complex with the ClpP peptidase. ClpX hydrolyzes ATP to generate mechanical force and translocate polypeptides through its central pore.

2003年3月1日 · Here, we report the identification of more than 50 E. coli proteins that are trapped in a ClpX-dependent fashion within an active-site mutant of ClpP. Analysis of these ClpXP substrates provides a more comprehensive understanding of the cellular roles of this protease and reveals five distinct classes of ClpX-recognition motifs.