2022年8月18日 · mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with low acid sensitivity. mCherry was derived from mRFP1.5 with the following mutations: N6D/K194N/T195V/D196N amino acid numbers relative to DsRed. show relative to mRFP1.5.

Students design and perform a safe and time-efficient random mutagenesis experiment using error-prone rolling circular amplification of a plasmid expressing the inducible fusion protein glutathione S-transferase (GST)-mCherry.

mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As an RFP, mCherry was derived from DsRed of Discosoma sea anemones, unlike green fluorescent proteins (GFPs) which are often derived from Aequorea victoria jellyfish. [1]

We have used a combination of protein design and directed evolution to develop mCherry variants with low cytotoxicity to Escherichia coli and altered excitation and emission profiles.

ABSTRACT: The 3-fold higher brightness of the recently developed mCherry-XL red fluorescent protein (FP) compared to its progenitor, mCherry, is due to a significant decrease in the nonradiative decay rate underlying its increased fluorescence quantum yield.

2022年12月27日 · The 3-fold higher brightness of the recently developed mCherry-XL red fluorescent protein (FP) compared to its progenitor, mCherry, is due to a significant decrease in the nonradiative decay rate underlying its increased fluorescence quantum yield.

2023年1月12日 · To examine the structural and dynamic role of the four mutations that distinguish the two FPs and closely related variants, we employed microsecond time scale, all-atom molecular dynamics simulations.

2016年10月17日 · To visualize and quantify mutations caused by genome-editing enzymes by using pmCherry-GFP, we designed primers to directionally clone the targeted region into the multi-cloning linker such...

2017年4月21日 · Students design and perform a safe and time-efficient random mutagenesis experiment using error-prone rolling circular amplification of a plasmid expressing the inducible fusion protein glutathione S-transferase (GST)-mCherry.

2022年8月10日 · mCherry2 was derived from mCherry with the following mutations: K92N/K138C/K139R/S147T/N196D/T202L amino acid numbers relative to DsRed. show relative to mCherry.