Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. …

Pair up words that look different but sound alike. The meaning of TRYPSINIZE is to subject to the action of trypsin. How to use trypsinize in a sentence.

Trypsin is a member of serine protease family frequently used to detach cells. The optimum activity for trypsin occurs at 37 °C, so pre-warmed trypsin is often used to speed up cell …

Generally, hES cells recover from trypsinization better when they are not dispersed to single cells but remain as small clumps of approximately 2–20 cells.

Trypsin, a proteolytic enzyme, is the standard way to detach adherent cell cultures and monolayers. This globular, pancreatic protease cleaves at the C-terminal side of lysine and …

Many have found that trypsinizing causes less death compared to scraping, as well as causes fewer changes in metabolic parameters (most likely because most of the cells are still intact).

2024年2月27日 · Proteolysis with the use of trypsin – or trypsinization – is a process where you expose cells to trypsin in oder to digest intercellular and cell-to-substrate linking proteins. The …

Failure to subculture confluent cells results in reduced mitotic index and eventually in cell death. The first step in subculturing is to detach cells from the surface of the primary culture vessel by …

2015年6月15日 · Trypsinizing is fine now because you won't be using these cells again. However, there are methods to calculate cell numbers without trypsinizing. Assuming that your flask is …

In this study, a triplicate 2D-DIGE strategy has been performed to monitor trypsin-induced proteome alterations. The differentially expressed spots were identified by MALDI-TOF MS …