Western blot analysis of extracts from various cell lines using SQSTM1/p62 Antibody. Cat. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. SQSTM1/p62 Antibody detects endogenous levels of total SQSTM1/p62 protein. Human, Mouse, Rat, Monkey.

Here, we present several protocols for monitoring autophagy-mediated degradation of p62 using Western blots, pulse-chase measurement of p62 half-life, immunofluorescence and immuno-electron microscopy, as well as live cell imaging with a pH-sensitive mCherry-GFP double tag.

Western blot is performed to observe the increase in autophagy protein after substance treatment in cells. It was confirmed that autophagy was increased by reduction of Beclin1 and p62.

I have been doing western blots for the autophagy cargo protein p62/SQSTM1. At certain time points, I see p62 appear as a doublet (as shown in the attached image), and other times it...

SQSTM1/p62 can be upregulated under different conditions, thus increasing the Western blot levels independently of any autophagic degradation. In some cases, autophagic stimuli can induce the expression of the SQSTM1/p62 gene and protein, as in the case of muscle atrophy induced by cancer.

Like LC3, western blotting analysis of p62 is only a snapshot of a dynamic process. For that reason, measurement of p62 in the presence and absence of lysosomal blockade provides essential information about the amount and rate of autophagic substrates sequestered and …

2020年5月19日 · To further verify that SiNP treatment increases the p62 protein level in vivo, we examined its expression through western blot analysis and found increased p62 accumulation in SiNP-treated mouse lung tissue (Fig. 1d).

2015年5月15日 · At present, the analysis of autophagic flux by Western blotting (WB), which measures two of the most important markers of autophagy, i.e., microtubule-associated protein 1 light chain 3 (LC3) and p62, is widely accepted in the scientific community.

We confirmed the mass spectrometry result by performing p62 immunoprecipitation followed by western blot for p62 and KEAP1 (Figure 1B) and in two additional sets of chronic IL-1 sublines that we generated (LNas2, LNbs2, LNas3, LNbs3; Figure S2). Thus, chronic IL-1 exposure reproducibly selects for cells that acquire high basal accumulation of ...