Huh-7 is an immortal cell line composed of epithelial-like, tumorigenic cells. The majority of Huh-7 cells show a chromosome number between 55 and 63 (mode 60) and are highly heterogeneous. These cells are adherent to the surface of flasks or plates and typically grow as 2D monolayers.

2012年4月4日 · Stable HepG2- and Huh7-based human hepatoma cell lines for efficient regulated expression of infectious hepatitis B virus. J. Hepatol. 45:636-645(2006) PubMed= 20215515 ; DOI= 10.1158/0008-5472.CAN-09-3458 ; PMCID= PMC2881662

HuH-7 Cells should be cultured in DMEM supplemented with 10% FBS. Cells should be grown at 37 °C in a 5% CO2 setting. To subculture: Renew medium 2-3 times a week, or as needed according to media pH, and maintain cell confluency between 30-90%.

2025年3月17日 · HuH7 cells are a widely used human hepatocellular carcinoma cell line that has significantly contributed to liver disease research, drug development, and virology studies. Their adaptability in culture and ability to support viral replication make them invaluable for studying hepatitis C virus (HCV) and other liver-related conditions.

Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium. Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.

To date, the human hepatoma cell line Huh7 and its derivatives is the only cell line that robustly replicates HCV. However, even different subclones and passages of this single cell line exhibit tremendous differences in HCV replication efficiency.

HuH-7 is a well-known cell line used as a model for investigating both hepatoma and HCV. Tumor cells acquire genetic alterations during their evolution, which underlie cancer development, progression and drug resistance [8].

We have discovered that a widely used hepatoma cell line, Huh-7, carries a HFE mutation similar to that associated with human HC. By HFE gene sequencing of Huh-7 genomic DNA, we found a TAC nucleotide deletion (c. 691_693del) responsible for loss of a tyrosine at position 231 (p. Y231del) of the HFE protein.

Human cell lines represent a valuable resource as in vitro experimental models. A hepatoma cell line, HuH-7 (JCRB0403), has been used extensively in various research fields and a number of studies using this line have been published continuously since it was established in 1982.

2019年11月1日 · Hepatic cell lines, such as Huh7, could provide a cost-effective alternative, however, they express negligible amounts of CYP450s and PXR. In this study, we show that dinaciclib, a potent...