RNA imaging with smFISH enables quantitative and qualitative gene expression experiments within single cells. With smFISH, researchers can measure cellular mRNA and perform spatial localization and quantification in locations such as the cytoplasm and nucleus.

单分子荧光原位杂交（smFISH）是分子生物学中用于检测RNA分子在细胞中定位和表达的技术。 它基于原位杂交技术，使用荧光标记的探针与目标RNA结合，形成荧光信号，通过显微镜观察可以观察到RNA的位置和数量。 与传统的原位杂交技术不同的是，smFiSh可以检测单个RNA分子，因此可以更准确地定量RNA的表达水平和定位。 此外，smFISH还可以对RNA进行多重染色，即 …

Here, we describe a single-molecule fluorescent in situhybridization (smFISH) method (as performed in Haimovich et al., 2017) that detects single RNA molecules in individual cells.

2022年10月24日 · Here, we present high-throughput smFISH (HT-smFISH), a simple and cost-efficient method for imaging hundreds to thousands of single endogenous RNA molecules in 96-well plates. HT-smFISH uses...

2015年4月9日 · Single-molecule fluorescence in situ hybridization (smFISH) has emerged as a powerful tool for studying the copy number and spatial organization of RNAs in single cells either in isolation or in their native tissue context (2, 3).

Single molecule fluorescence in situ hybridization (smFISH) is a powerful technique to study gene expression in single cells due to its ability to detect and count individual RNA molecules.

2024年3月20日 · FISH is a powerful technique which allows the visualization of mRNA molecules at the subcellular level in fixed samples, via probing with fluorescent oligos using widefield fluorescence microscopy.

2025年3月15日 · Explore the principles and methods of smFISH for precise single-molecule RNA detection, including probe design, sample preparation, and imaging techniques. Detecting individual RNA molecules within cells provides valuable insights into …

2018年5月25日 · Single molecule fluorescence in situ hybridization (smFISH) is a powerful technique to study gene expression in single cells due to its ability to detect and count individual RNA molecules.

2018年11月5日 · Here, we describe a single-molecule fluorescent in situ hybridization (smFISH) method (as performed in Haimovich et al., 2017 ) that detects single RNA molecules in individual cells.