RDE-1 (RNAi-DEfective 1) is a primary Argonaute protein required for RNA-mediated interference (RNAi) in Caenorhabditis elegans. The rde-1 gene locus was first characterized in C. elegans mutants resistant to RNAi, and is a member of a highly conserved Piwi gene family that includes plant, Drosophila, and vertebrate homologs. [1]

1999年10月15日 · Two loci, rde-1 and rde-4, are defined by mutants strongly resistant to RNAi but with no obvious defects in growth or development. We show that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates.

Although null mutants lacking rde-1 show no phenotypic response to dsRNA, the amount of siRNAs generated from an injected dsRNA trigger was comparable to that of wild-type. By contrast, mutations in rde-4 substantially reduced the population of siRNAs derived from an injected dsRNA trigger.

2019年2月20日 · Here we constructed a germline-specific RNAi strain by combining an indel mutation of rde-1, which encodes an Argonaute protein that functions cell autonomously to ensure RNAi efficiency,...

In C. elegans, RDE-1, RDE-4 and RRF-1 (a putative RdRP) are required for dsRNA-induced silencing in the soma, even when the trigger dsRNA is expressed from a transgene in the nuclei of target tissues [16,19,36].

2009年1月18日 · Here we show that RNase H activity of RDE-1 is required only for efficient removal of the passenger strand of the siRNA duplex and not for triggering the silencing response at the target-mRNA...

2020年11月5日 · We discovered that rde-1(ne219), a commonly used RNAi-resistant mutant strain, retains considerable RNAi capacity against RNAi directed at PUFA synthesis genes.

2021年7月28日 · For tissue-specific RNAi assays, we used either rde-1 or sid-1 mutant worms in which wild-type rde-1 or sid-1 expression has been rescued using tissue-specific promoters, respectively. Tissue...

Strain: WM118, Genotype: rde-1 (ne300) V; neIs9 X., Description: neIs9 [myo-3::HA::rde-1 + rol-6 (su1006)] X. Transgene rescues muscle RNAi defect. Rollers.

1999年10月15日 · Two loci, rde-1 and rde-4, are defined by mutants strongly resistant to RNAi but with no obvious defects in growth or development. We show that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates.

2002年2月7日 · Among the C. elegans genes required for RNAi are rde-1 and rde-4, which have no readily detectable mutant phenotype other than resistance to RNAi . These mutants are resistant to dsRNA targeting both somatic and germ line–specific genes and are also resistant to dsRNA produced by transgenes .

2022年7月1日 · Primary AGOs that feed into nuclear RNAi pathways include: RDE-1, binds primary sRNAs derived from Dicer products; PRG-1, binds piRNAs; and ERGO-1, binds 26G-RNAs. Nuclear WAGOs translocate to the nucleus (likely dependent on 22G-RNA binding) where they can engage nascent transcripts and recruit nuclear co-factors such as the NRDE complex …

Here we show that the C. elegans RNAi pathway gene, rde-4, encodes a dsRNA binding protein that interacts during RNAi with RNA identical to the trigger dsRNA. RDE-4 protein also in- teracts in vivo with DCR-1, RDE-1, and a conserved DExH-box helicase.

Previous studies have shown that, in Caenorhabditis elegans, a complex containing the enzymes Dicer and the Argonaute RDE-1 process dsRNA. Dicer is responsible for cleaving dsRNA into …

The frSi17 insertion can be detected using a primer within the mtl-2 promoter (jep1061: aacaaacgtgggatgtaacc) in combination with downstream primer in rde-1 (jep2817 tcatactcgtagtattcccg), producing a 786 bp product if insertion is present.

These findings and our genetic studies suggest that RDE-12 is first recruited to target mRNA by upstream AGOs (RDE-1 and ERGO-1), where it promotes small RNA amplification and/or WAGO-1 loading.

AGO1 is similar to QDE-2 required for quelling and RDE-1 required for RNAi. Sequencing of ago1 mutants revealed one amino acid essential for PTGS that is also present in QDE-2 and RDE-1 in a highly conserved motif.

2000年10月10日 · AGO1 is similar to QDE-2 required for quelling and RDE-1 required for RNAi. Sequencing of ago1 mutants revealed one amino acid essential for PTGS that is also present in QDE-2 and RDE-1 in a highly conserved motif.

In animals lacking RDE-1, the introduction of double-stranded RNA does not trigger any detectable level of RNAi. Here we show that RNase H activity of RDE-1 is required only for efficient...

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RTÉ Radio 1 (Irish: RTÉ Raidió 1) is an Irish national radio station owned and operated by RTÉ and is the direct descendant of Dublin radio station 2RN, which began broadcasting on a regular basis on 1 January 1926.. The total budget for the station in 2010 was €18.4 million. [1] It is the most-listened-to radio station in Ireland. [2]

Strain: VP303, Genotype: rde-1 (ne219) V; kbIs7., Description: kbIs7 [nhx-2p::rde-1 + rol-6 (su1006)]. Rollers. RNAi effective only in intestine. Maintain under normal conditions. Reference: Espelt MV, et al., J Gen Physiol. 2005 Oct;126 (4):379-92.

2025年3月18日 · A 29-year-old man has been charged with a number of counts of criminal damage at Dublin Airport yesterday. Lukas Kaunietis, of no fixed abode, was arrested in the airport's Terminal 1 building ...

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2025年3月16日 · Derry manager Paddy Tally believes that his side will be able to rebound from relegation to Division 2 of the Allianz Football League, and is confident his players will recover in time for the ...