2024年12月25日 · ( rpb1_human,p24928) (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicase and transcriptase for the viral RNA circular genome.

RNA polymerase II (RNAP II) consists of 12 subunits and transcribes all mRNA and many noncoding RNA genes. Rpb1, the largest subunit, possesses a unique C-terminal domain (CTD) that consists of tandem heptad repeats, with a consensus sequence of Tyr-Ser-Pro-Thr-Ser-Pro-Ser (Y 1 S 2 P 3 T 4 S 5 P 6 S 7). The number of repeats, generally ...

The C-terminal domain (CTD) of Rpb1, the largest subunit of RNA polymerase II (RNApII), coordinates recruitment of RNA- and chromatin-modifying factors to transcription complexes. It is unclear whether the CTD communicates with the catalytic core ...

2020年10月28日 · Stacc–seq can also detect Pol II binding using as few as 500 mESCs, using antibodies against RPB1 (the largest subunit of Pol II), regardless of the phosphorylation state of the CTD (Extended ...

2018年5月30日 · Here we identify a histidine-rich domain in cyclin T1 that promotes the hyperphosphorylation of the CTD and stimulation of transcription by CDK9. The histidine-rich domain markedly enhances the...

RPB1 is the catalytic subunit of RNA polymerase II, which is responsible for the transcription of messenger RNAs (mRNAs) and most small nuclear RNAs and microRNAs in the nucleus (8).

2019年11月15日 · Silencing RPB1 by genetic technology led to a potent regression of human refractory AML in mouse models. These findings reveal that dysregulated RPB1 is a central oncogenic hub that drives overgrowth by hijacking an array of …

2019年8月1日 · We engineered strains of the yeast Saccharomyces cerevisiae for rapid, specific inactivation of individual kinases by addition of a covalent inhibitor. While effective, the sensitized kinases can display some idiosyncrasies, and inhibition can be surprisingly transient.

2022年10月20日 · The results showed that RPB1 (CTD), RPB3, RPB5, and RPB8 depletion significantly increased the readthrough signals, but the changes were not significant for RPB9 and RPB11 and RNA-seq signals decreased for RPB2, RPB6, RPB7, and RPB10 at polyadenylation sites (Figures 5 B and S7 B).

2023年3月1日 · The Flag epitope tag was added to the N terminus of the first RNAPII subunit (RPB1) in DPY30–mAID degron cells (RPB1–APEX2 cells).