The LX-2 cell line retains key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis.

With increasing interest in developing antifibrotic therapies, there is a need for cell lines that preserve the in vivo phenotype of human HSCs to elucidate pathways of human hepatic fibrosis. We established and characterised two human HSC cell lines termed LX-1 and LX-2, and compared their features with those of primary human stellate cells.

LX-2 cells are used to examine the cellular and molecular mechanisms underlying liver fibrosis, including the role of stellate cells in extracellular matrix deposition and the modulation of these processes by therapeutic agents.

The LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, …

RNA sequencing was used to quantitate the transcriptomic changes induced in LX-2 cells, an activated human HSC line, following TGF-b1 treatment. In total, 5,258 genes were found to be significantly differentially expressed with a false discovery rate cut-off of < 0.1.

Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous.

2018年10月25日 · Herein, we use quantitative proteomics and ingenuity pathway analysis to show that transglutaminase 2 (TGM2) is upregulated in the course of activated hepatic stellate cells promoting...

Stellate cells, including the immortalized stellate cell line LX-2, retain cytokine signaling and express the major receptors for interleukin (IL)-6, tumor necrosis factor (TNF)- α and IL-1 β in culture (Xu et al., 2005; Tarrats et al., 2011; Harvey et al., 2013).

Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells. Proteomic analysis of extracellular matrix from the hepatic stellate …

2013年10月8日 · Our findings indicate that LX-2 cells comprise a complex karyotype with various structural abnormalities. However, the invariant STR signature and defined specific translocations could be established for LX-2 cells.