SHED-TK demonstrated a strong bystander effect in vitro and in vivo and completely eradicated H1299 NSCLC in the brain. SHED-TK cells implanted intratumorally followed by GCV administration significantly suppressed the growth of H1299 and improved survival time.

1995年6月12日 · The herpes simplex virus thymidine kinase (HSV-TK) converts ganciclovir (GCV) into a toxic product and allows selective elimination of TK+ cells in vitro and in vivo. It is currently being used in clinical gene therapy trials as a therapeutic gene or as a safety marker.

The use of pro-drug-activating genes is a promising approach for cancer gene therapy, especially herpes simplex virus thymidine kinase gene (HSV-TK) in combination with ganciclovir (GCV), which is currently used in gene therapy-based experimental trials for cancer treatment, and in clinical treatment of brain tumors[7,8].

Herpes Simplex Virus type 1 (HSV-1) thymidine kinase (TK) is currently the most widely used suicide agent for gene therapy of cancer. Tumor cells that express HSV-1 thymidine kinase are rendered sensitive to prodrugs due to preferential phosphorylation by this enzyme.

2011年8月23日 · The most widely used suicide genes are Herpes Simplex Virus-1 Thymidine Kinase (HSV-TK), and Cytosine Deaminase (CD) from the virus Herpes simplex or the bacterium Escherichia coli respectively [7]. Prior research has focused on the mechanism of initiation and development of tumors.

Herpes simplex virus type 1 (HSV) thymidine kinase (TK) has been widely used in suicide gene therapy for the treatment of cancer due to its broad substrate specificity and the inability of the endogenous human TK to phosphorylate guanosine analogs such as ganciclovir (GCV).

Herpes simplex virus (HSV) thymidine kinase (TK) expression and the HSV TK gene have been evaluated in studies of gene control, as well as in animal and human studies of viral pathogenesis, including HSV latency.

2006年2月3日 · Here a new optimized derivative of the herpes simplex virus 1-thymidine-kinase (HSV1-TK) gene is described. The HSV-TK gene is frequently used in experimental studies on gene-directed...

Thymidine kinase from herpesvirus is a sub-family of thymidine kinases that catalyses the transfer of phospho group of ATP to thymidine to generate thymidine monophosphate, which serves as a substrate during viral DNA replication. [2] [3]Its presence in herpesvirus-infected cells is used to activate a range of antivirals against herpes infection, and thus specifically target the therapy ...

The herpes simplex virus type 1 (HSV-1)-encoded thymidine kinase (TK) appears to be such a protein. HSV-1 TK is a multifunctional enzyme that possesses kinase activities normally performed by three separate enzymes.

1999年6月1日 · The herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene promoter contains binding sites for the cellular transcription factors such as Spl, CTF, and TFIID, each of which affects basal level expression of the TK gene.

2025年3月20日 · To deplete male germ cells using HSV-TK, we inserted the P2A self-cleaving peptide and HSV-thymidine kinase (HSV-TK) gene into the C-terminus of the germ cell-specific gene, Vasa (a.k.a. Ddx4 or ...

Herpes simplex virus (HSV) thymidine kinase promoter. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different AflIII sites may not be compatible. ApoI is typically used at 50°C, but is 50% active at 37°C. Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.

2013年1月2日 · On the county map Fawn Creek can be found in Township 35-south and Range 15-east. On the township map Fawn Creek can be found un section 6. By 1916 the town ship map shows that Tyro moved a mile north and is now in section 31.

The herpes simplex virus type 1 thymidine kinase (HSV-TK) gene, in combination with ganciclovir, is the most widely used enzyme/prodrug suicide system from basic research to clinical applications. In the present study, we attempted to establish human iPSCs that stably expressed HSV-TK with either lentiviral vectors or CRISPR/Cas9-mediated ...

Review maps and data for the neighborhood, city, county, ZIP Code, and school zone. July 1, 2024, data includes home values, household income, percentage of homes owned, rented or vacant, etc. Quick & Easy Ways to... Township of Fawn Creek: maps, driving directions and local area information.

Overview of Township of Fawn Creek, Montgomery County, Kansas, United States -- Histopolis.com - Collaborative Genealogy and History.

HSV resistance to ACV has been associated with mutations occurring either in the viral TK gene or the viral DNA polymerase gene. Three mechanisms of resistance have been proposed: a loss of TK activity (TK-deficient virus), an alteration of TK substrate specificity (TK-altered virus), and/or an alteration of DNA polymerase activity (15).

2021年4月28日 · It only shows up in Fawn Creek Kansas. This question is locked and replying has been disabled. Community content may not be verified or up-to-date. Learn more. Thanks …