2023年3月15日 · The GCaMP6 (ref. 5) and jGCaMP7 (ref. 6) sensors enable detection of single action potentials (APs) under favourable conditions and are often used to monitor the activity of large groups of ...

2013年7月17日 · Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons...

2023年2月22日 · GCaMP6 sensors can detect individual action potentials with high reliability and can be used to image large populations of neurons as well as tiny synaptic compartments such as spines and terminals (Chen et al., 2013). These indicators have become the most popular calcium sensors since their creation in 2013.

Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and peripheral nervous system under the control of the Thy1.2 promoter. These transgenic lines exhibit stable and layer-specific expression of GCaMP6 in …

Our detailed spectroscopic study reveals the simplest explanation for how GCaMP6m changes fluorescence in response to Ca 2+ is with a four-state model, in which a Ca 2+ -dependent change of the chromophore protonation state, due to a shift in pK a, is the predominant factor.

2023年8月28日 · Single-fluorophore genetically encoded calcium indicators (GECIs), such as GCaMP, are widely used tools to investigate neuronal activity. Their primary advantage lies in their capability to provide real-time and highly sensitive responses to fluctuations in intracellular Ca2+ concentrations.

2013年7月18日 · Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo.

2016年12月6日 · We propose that the design strategy used for generating GCaMP6f u is applicable for the acceleration of the response kinetics of GCaMP-type calcium indicators. Genetically encoded Ca 2+...

Two such alternative methods with cellular scale resolution have matured in the last few decades and will be reviewed here: the transcription of immediate early genes, foremost c-fos, and the influx of calcium into neurons as reported by genetically …

Because the neutral and anionic form of GCaMP6 have different absorption and fluorescence excitation spectra, the relative fluorescence change (i.e., ΔF/F) of GCaMP6 upon binding to calcium ion depends strongly on the excitation wavelength.