Calu-3 is a cell line obtained from a male, lung adenocarcinoma patient that can be used in cancer and toxicology research.

Calu-3 is a human lung cancer cell line commonly used in cancer research and drug development. Calu-3 cells are epithelial and can act as respiratory models in preclinical applications. [1] Calu-3 cells were first derived in 1975 by Germain Trempe and Jorgen Fogh of the Memorial Sloan Kettering Cancer Center.

This article reviews the application of the human airway Calu-3 cell line as a respiratory model for studying the effects of gas concentrations, exposure time, biophysical stress, and biological agents on human airway epithelial cells.

2020年10月22日 · Calu-3 cells are a submucosal gland cell line, which was generated from a bronchial adenocarcinoma. Under air–liquid interface conditions Calu-3 cells grow in monolayers, producing mucus and cilia-like microvilli.

Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene.

Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl-secretion. Demonstration of an interferon gamma-dependent tumor surveillance system in immunocompetent mice. Increased dosage and amplification of the focal adhesion kinase gene in human cancer cells.

Calu-3 cells constitute a human-derived airway epithelial cell line representing non-small cell lung carcinoma. They are widely used in biomedical research that includes studying lung cancer biology, respiratory diseases, host-pathogen interactions, and drug transport in the airway.

The Calu-3 tumor model is a well-established xenograft model to study the effects therapeutics, such as erlotinib, trastuzumab and pertuzumab on HER2 and EGFR expressing cells. Calu-3 xenografts are commonly used to investigate the effects of various drugs and compounds on tumor growth and metastasis.

The Calu-3 CDX mouse model enables investigation of human lung cancer cell therapeutics targeting migration, apoptosis, anti-angiogenesis and anti-tumor growth in a human-similar tumor environment. Such tumor growth delay studies include the combination studies of erlotinib and cetuximab, or trastuzumab and pertuzumab.

Calu-3 cells formed polarized monolayers with tight junctions and Rte of approximately 100 omega.cm2. Baseline Isc was approximately 35 microA/cm2 and was increased by approximately 75 microA/cm2 on elevation of intracellular adenosine 3',5' …

The results show that Calu-3 fully polarize when grown on permeable supports as liquid-covered cultures. Polarized Calu-3 are susceptible to RSV infection and release infectious virus primarily from the apical surface, consistent with studies in NHBE cells.

2021年3月23日 · Here, we developed a 3D model of the human bronchial epithelium using Calu-3 cell line and demonstrated its viability and functionality for 21 days without subculturing.

Calu-3 cells exposed to graded oxygen concentrations demonstrate cell dysfunction and inflammation in a dose-dependent manner. Modeling positive airway pressure reveals that pressure may exert a greater injurious effect and cytokine response than oxygen.

In this study, we report differences in response of Calu-3 epithelial cells to exogenous stimuli Staphylococcus aureus enterotoxin B (SEB) and house dust mite (HDM) extract compared to primary nasal epithelial cells.

The Calu-3 cell line can assist in finding potential drug therapies and has shown to be useful in mimicking in vivo conditions.3 This similarity has allowed for many pharmaceutical applications such as drug transport, studying aerosol deposition, controlled release studies, and identification of potential drug-drug interactions.4 The ...

2010年6月27日 · This article reviews the application of the human airway Calu-3 cell line as a respiratory model for studying the effects of gas concentrations, exposure time, biophysical stress, and biological agents on human airway epithelial cells. Calu-3 cells are grown to confluence at an air-liquid interface on permeable supports.

Cell culture models such as Calu-3 provide a simple and reproducible system to study the underlying mechanisms by which inhaled therapeutics interact with the lungs.

2015年3月10日 · In this study we have investigated the effects of different cell culture conditions on the Calu-3 epithelial cell model. Calu-3 cells were cultured in media A-MEM at the air–liquid (A–L) or liquid–liquid (L–L) interface for one or three wks (weeks).

CALU-3 cells cultivated onto CellFate® resulted in a pseudostratified organization, a typical morphology of the human respiratory tract epithelium. The current model opens perspectives for studies involving physiological characterization, improving its relevance for the understanding of the pathophysiology of diseases as well as the response ...