2007年8月13日 · In this review, we will discuss what we have learned from the structures and related biochemistry of HATs and HDACs and the implications of these findings for the design of protein effectors to...

Histone acetylation marks are written by histone acetyltransferases (HATs) and read by bromodomains (BrDs), and less commonly by other protein modules. These proteins regulate many transcription-mediated biological processes, and their aberrant activities are correlated with several human diseases.

2003年12月19日 · NAD+ analogues including beta-2'-deoxy-2'-fluororibo-NAD+ and a His-to-Ala mutant were used to probe the mechanism of nicotinamide-ribosyl cleavage and acetyl group transfer. We demonstrate that nicotinamide-ribosyl cleavage is distinct from acetyl group transfer to the 2'-OH ribose.

In this review, we will focus on reciprocal relationships between the epigenome and metabolism, mainly using histone methylation and acetylation as examples, and discuss how cellular SAM and acetyl-CoA levels affect chromatin states.

Acetyl-CoA represents a key node in metabolism due to its intersection with many metabolic pathways and transformations. Emerging evidence reveals that cells monitor the levels of acetyl-CoA as a key indicator of their metabolic state, through ...

2023年9月22日 · For example, histone acetyltransferases (HATs) promote acetylation of histone lysine residues using the endogenous thioester acetyl reagent acetyl-CoA (Ac-CoA) and regulate the histone...

2018年12月5日 · Deacetylation is the removal of an acetyl group and occurs on a plethora of targets and for a wide range of biological reasons. Several pathogens deacetylate their surface carbohydrates to...

2023年5月3日 · Using stable isotope tracing, we show that both glucose and fatty acids contribute to acetyl-CoA pools and histone acetylation in DKO cells and that acetylcarnitine shuttling can transfer two-carbon units from mitochondria to cytosol.

2024年5月17日 · Acetyl-CoA synthetase short-chain family member 1 (ACSS1; previously known as AceCS2) catalyzes the ligation of acetate and CoA to generate acetyl-CoA in the mitochondrial matrix, enabling the utilization of acetate for energy during conditions of nutrient deprivation .

To gain a better understanding of the catalytic mechanism and of the biological implications of producing this molecule, we have performed a detailed enzymatic and structural characterization of O -acetyl-ADP-ribose.