In the present study, we aimed to clarify the validity of using U251 glioma cells as a source of GSC culture and critically evaluate the heterogeneous stem-like phenotypes of these cells when grown under various culture conditions.

Purchase high quality cryopreserved human malignant glioblastoma multiforme (GBM) U251 cells from ECACC that contain cancer stem cells for brain cancer research

HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique. Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5.

U-251 MG Glioblastoma Cell Line that expresses EGFR and contains GFAP positive cells. Buy Here!

2022年8月3日 · Unlike the U87 model, U251 cells recapitulate many of the main glioblastoma characteristics observed in patients. U251-RedFLuc cells grew slowly and formed small tumors. For more significant tumor growth and a median survival similar to that in U87-luc2 mice, more tumor cells need to be implanted.

The U-251 MG cell line is a well-characterized human glioblastoma multiforme (GBM) cell line that is extensively used in neuro-oncology research.

Here, we developed a novel method for the 3D cultivation of the human glioblastoma cell line U251 within the nanofibrillar cellulose hydrogel, GrowDex®. We demonstrate that 3D cultivation of U251 cells in GrowDex results in an increased cell viability.

Methods: We studied the MGMT-methylated GBM cell line U251 and its in vitro derived TMZ-resistant subline, U251/TMZ-R. Cytotoxicity of TMZ, CCNU, and radiation was tested. Both cell lines were analyzed for MGMT promotor status and expression of mismatch repair genes (MMR).

Through functional experiments, we evaluated the interrelationship among IGF2BP2, DANCR, phosphotyrosine interaction domain containing 1 (PID1), and forkhead box protein O1 (FOXO1) and further assessed their impact on the sensitivity of GBM cells to etoposide.

U251 MG is a cell line derived from human glioblastoma multiforme, a highly aggressive malignant brain tumor. The U251 MG human cell line was derived from a 75-year-old brain cancer patient diagnosed with glioblastoma astrocytoma.

U-251 MG cells are highly amenable to transfection, and a variety of methods can be used effectively, including lipofection, electroporation, and viral transduction. Lipofection is particularly popular due to its simplicity and high efficiency.

2015年5月8日 · Single nucleotide polymorphism (SNP) array analysis has demonstrated that the SNB-19 and U251 lines are derived from the same individual. (Garraway LA, et al. Integrative genomic analyses identify MITF as a lineage survival oncogene amplified in malignant melanoma.

2014年1月31日 · In this study, we investigated the possible upregulated expression of SLC22A18-induced enhancement of radiosensitivity of human glioma U251 cells in order to provide evidence in support of...

In the present study, we aimed to clarify the validity of using U251 glioma cells as a source of GSC culture and critically evaluate the heterogeneous stem-like phenotypes of these cells when grown under various culture conditions.

U251 cells were transduced with CMV-Luc baculovirus in the presence of heparin. One day later, luciferase activities of the transduced cells were measured and compared to those without heparin treatment.

2014年8月1日 · In this report, we present a thorough characterization of the commonly used glioblastoma (GBM) model U-251, which in numerous publications has been wrongly identified as U-373, due to an...

2016年11月1日 · We examined the active histone marks H3K4Me3 and H3K9Ac in BLM, A375, T98G (bearing -146C>T mutation in TERT promoter), and U251 (bearing -124C>T mutation in TERT promoter) cell lines up to -5 kb upstream of the TERT gene.

The present study established a novel method for obtaining CD133 (+) and CD133 (−) U251 cells. In the current study, gene recombination technology was successfully used to construct two types of gene expression vectors, which were stably transfected in the U251 cell line.

U251 cells were transduced with CMV-Luc baculovirus in the presence of heparin. One day later, luciferase activities of the transduced cells were measured and compared to those without heparin treatment.

In vitro and in vivo experiments revealed that NP3 effectively delivered ASOs to the brain, resulting in a marked reduction in TGF-β2 mRNA and protein expression in U251 glioma cells. This nanoparticle system showed promising anti-cancer effects and extended circulation time, highlighting its potential for targeted brain therapies.