Ribonuclease H (abbreviated RNase H or RNH) is a family of non- sequence -specific endonuclease enzymes that catalyze the cleavage of RNA in an RNA/ DNA substrate via a hydrolytic mechanism.

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. There are two main types of RNases H, and at least one of them is present in most organisms.

RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA, when hybridized to DNA. Recombinant enzyme supplied with Reaction Buffer

RNase H is a ubiquitous enzyme that degrades the RNA strand of an RNA–DNA duplex. It has been identified in organisms as diverse as viruses and human cells (Crouch and Dirksen, 1985). At least two classes of RNase H have been identified in eukaryotic cells.

This review summarizes the mechanisms of substrate recognition and enzymatic cleavage by different classes of RNases H with particular insights into structural features of nucleic acid binding, specificity towards RNA and/or DNA strands and catalysis.

2019年12月1日 · RNases H are a family of endonucleases that hydrolyze RNA residues in various nucleic acids. These enzymes are present in all branches of life, and their counterpart domains are also found in reverse transcriptases (RTs) from retroviruses and retroelements.

2024年7月5日 · Ribonuclease H (RNase H) is a critical enzyme found in various organisms, ranging from bacteria to humans. Its ability to selectively degrade the RNA strand in RNA-DNA hybrids makes it indispensable for numerous biological processes, including DNA …

The RNase H domains of M-MLV and HIV-1 reverse transcriptases are structurally very similar to the Escherichia coli and Bacillus halodurans RNases H, and to human RNase H1, and these similarities provide key insights concerning substrate recognition and catalysis by …

The polymerization-independent RNase H activity participates in removal of genomic RNA that remains annealed to the minus-strand DNA.

Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5' phosphateterminated oligoribonucleotides and single-stranded DNA.