Photobleaching - Wikipedia
In optics, photobleaching (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce. This is caused by cleaving of covalent bonds or non-specific reactions between the fluorophore and surrounding molecules.
Photobleaching Principles | Thermo Fisher Scientific - US
What is photobleaching? A fluorophore can repeatedly undergo the fluorescence process—in theory, indefinitely. This is extremely useful, because it means that one fluorophore molecule can generate a signal multiple times.
Photobleaching - Olympus - Life Science
The phenomenon of photobleaching (also commonly referred to as fading) occurs when a fluorophore permanently loses the ability to fluoresce due to photon-induced chemical damage and covalent modification.
Photobleaching - an overview | ScienceDirect Topics
Photobleaching is a process in which, following repeated excitation, a fluorophore is irreversibly destroyed. Because photobleaching causes a loss of fluorescence signal, most quantitative fluorescence microscopy measurements are made under conditions where photobleaching is …
Photobleaching - an overview | ScienceDirect Topics
Photobleaching —the irreversible photochemical destruction of a fluorophore—leads to two kinds of artifacts on different timescales. (1)The sudden disappearance of a fluorophore results in shorter residence times in the detection volume and apparent faster diffusion.
How to Minimize Photobleaching in Fluorescence Microscopy: A ...
Photobleaching is a common challenge in fluorescence microscopy that can significantly affect the quality and reliability of imaging experiments. It occurs when fluorescent molecules (fluorophores) lose their ability to emit light after prolonged exposure to intense illumination. This article explores practical strategies to prevent or minimize ...
What is photobleaching, and how does it impact medical imaging?
2024年10月2日 · Photobleaching happens when fluorophores, the molecules responsible for fluorescence, are exposed to continuous or intense light. Photochemical alterations cause permanent changes to the molecule, making it lose its ability to fluoresce.
What Is Photobleaching, and How Does It Impact Medical Imaging?
2024年9月30日 · Photobleaching is a major challenge in medical imaging, as it compromises the accuracy of fluorescent-based techniques. See how photobleaching works and find out how you can overcome this challenge to help medical applications …
Super-photostable organic dye for long-term live-cell single …
3 天之前 · Phoenix Fluor 555 (PF555) is a bright dye with an exceptional, order-of-magnitude longer photobleaching lifetime than conventional organic dyes that enables extended live-cell single-molecule ...
Photobleaching in Fluorescence Microscopy - AZoLifeSciences
2021年2月1日 · In this case, reducing the time of exposure to light will reduce the number of times the fluorophores undergo the excitation-emission cycles, thus reducing the photobleaching. This can be achieved by reducing the pixel dwell of laser …