MB49 mouse urothelial carcinoma cell line is widely used to generate in vitro and in vivo models of bladder cancer. Transformed cells may be transplanted into animal models.

2019年4月1日 · The MB49 murine bladder carcinoma model can be employed as a robust preclinical immuno-oncology model. Our data supports the use of this tool in investigating novel treatment combinations with checkpoint inhibitors or costimulatory molecules.

2019年5月15日 · Recent data suggest that patients with a basal/stem-like bladder cancer (BC) subtype tend to have metastatic disease, but this is unconfirmed. Here we report the identification of murine MB49...

Recent data suggest that patients with a basal/stem-like bladder cancer (BC) subtype tend to have metastatic disease, but this is unconfirmed. Here we report the identification of murine MB49 cell line sub-clones with stem-like characteristics in culture.

Here, we have characterized immune cell infiltration and chemoattractant molecules in mouse orthotopic MB49 bladder tumors. Our data show a 100-fold increase in CD45 + immune cells from day 5 to day 9 tumors including T cells and mainly myeloid cells.

MB49 is a urothelial carcinoma cell line derived from a male C57BL/6 mouse by exposing primary bladder cells to DMBA (7,12-dimethylbenz [a]anthracene) for 24 hours and subsequent culturing. The cell line, despite its male origin, lacks the Y-chromosome and …

MB49, a mouse urothelial carcinoma cell line is widely used as an in vitro and in vivo model of bladder cancer.

As described below, MB49 is considered to have a cold tumor phenotype with very low levels of CD8+ and CD4+ T cell infiltration (<3%) and significant presence of immunosuppressive myeloid populations (~65%)

MB49 cell line can be used both as an in vitro and in vivo murine model for bladder cancer, thanks to its clinically relevant metastatic potential. MB49 cells also shares pivotal tumour characteristics with human bladder cancer.

MB49 cells are a urothelial carcinoma line derived from an adult C57BL/6 mouse by exposure of primary bladder epithelial cell explants to 7,12-dimethylbenz[a]anthracene (DMBA) for 24 hr followed by long-term culture.