2023年3月15日 · The GCaMP6 (ref. 5) and jGCaMP7 (ref. 6) sensors enable detection of single action potentials (APs) under favourable conditions and are often used to monitor the activity …

2013年7月17日 · Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors …

2023年2月22日 · GCaMP6 sensors can detect individual action potentials with high reliability and can be used to image large populations of neurons as well as tiny synaptic compartments …

Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and peripheral nervous system under the control of the Thy1.2 promoter. …

Our detailed spectroscopic study reveals the simplest explanation for how GCaMP6m changes fluorescence in response to Ca 2+ is with a four-state model, in which a Ca 2+ -dependent …

2023年8月28日 · Single-fluorophore genetically encoded calcium indicators (GECIs), such as GCaMP, are widely used tools to investigate neuronal activity. Their primary advantage lies in …

2013年7月18日 · Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of …

2016年12月6日 · We propose that the design strategy used for generating GCaMP6f u is applicable for the acceleration of the response kinetics of GCaMP-type calcium indicators. …

Two such alternative methods with cellular scale resolution have matured in the last few decades and will be reviewed here: the transcription of immediate early genes, foremost c-fos, and the …

Because the neutral and anionic form of GCaMP6 have different absorption and fluorescence excitation spectra, the relative fluorescence change (i.e., ΔF/F) of GCaMP6 upon binding to …