Ex vivo confocal microscopy (EVCM) is a an emerging imaging technique that allows real-time microscopic examination of freshly excised cutaneous tissue that eliminates the need for standard embedding, processing, and sectioning that is necessary for conventional histology [1].

Confocal microscopy allows noninvasive high-resolution imaging of fresh tissue with or without using fluorescent dyes as an external contrast agent.

Recent developments in ex vivo confocal microscopy allow for faster pathology assessment with greater accuracy by the visualization of cellular and architectural details, similarly to standard pathology, in either paraffin-embedded or frozen samples.

Vivascope 2500 ex vivo confocal microscopy (EVCM) is an innovative device that allows rapid, real‐time, quasi histologic, microscopic examination of excised cutaneous tissue within a few minutes without frozen sectioning or traditional tissue processing, embedding and sectioning.

Ex vivo imaging of tissues can be performed by using various types of optical imaging techniques. These next-generation digital microscopy tools have a promising potential for utilization in surgical pathology practice.

2020年4月1日 · The ex vivo confocal microscope is an imaging system designed to analyze freshly excised tissue using two diode lasers with different wavelengths. The technique can dramatically reduce margin analysis times and offers a sensitivity of 88% and a specificity of 89% relative to histopathology.

2022年4月30日 · Ex vivoconfocal microscopy (EVCM) acquires images in two modes: reflectance confocal microscopy (RCM) and fluorescence confocal microscopy (FCM).

Details the latest advances in the ex vivo confocal microscopy techniques; Features a practically applicable step-by-step guide to ex-vivo image acquisition; Provides a variety of image types for each condition covered to assist diagnostic skill acquisition

2020年3月5日 · Recent developments in ex‐vivo CM allows for faster pathology assessment with greater accuracy by the visualisation of cellular and architectural details similar to standard pathology either in paraffin embedded or frozen samples. They include the possibility of multimodal confocal microscopy using different lasers and fusion images.

2018年4月30日 · This review aims to give an overview of the current available applications of ex vivo confocal microscopy (EVCM) in dermatology. EVCM is a relatively new imaging technique that allows microscopic examination of freshly excised unfixed tissue.