Learn how to activate T cells with functional antibodies CD3 and CD28 with this detailed protocol.

We want to make it easier to activate and expand human T cells while still maintaining a high viability of these cells. That's why we developed ImmunoCult™ Human CD3/CD28 T Cell Activator. Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens.

2014年1月27日 · This has been developed primarily in the context of cell interaction with extracellular matrix through integrins, but recent studies showed that T cells exhibit mechanosensing through CD3 and CD28, nonintegrin receptors that provide activation and costimulatory signals.

T Cell TransAct™ consists of a colloidal polymeric nanomatrix conjugated to humanized CD3 and CD28 agonists providing primary and co-stimulatory signals for an optimized and efficient T cell activation and expansion.

We engineered two recombinant cross-interacting Bs-Abs (CriBs-Abs) by incorporating a peptide tag and its cognate single-chain variable fragment (scFv), respectively, into a pair of (tumor × CD3) and (tumor × CD28) binding Bs-Abs.

CD28 is critical for regulatory T cell survival and the maintenance of immune homeostasis. We outline the roles that CD28 and its family members play in human disease and we review the clinical efficacy of drugs that block CD28 ligands.

Analogous to using similar or identical CD3-binding domains in multiple CD3 TCEs, the same monovalent CD28-binding domain in XmAb808 can be readily coupled with binding domains for other tumor-specific antigens to generate new clinical candidates against a range of cancers . We incorporated a bivalent B7-H3–binding domain in XmAb808 to drive ...

2020年1月8日 · Here, we introduce a class of bispecific antibodies that mimic signal 2 by bridging TSA to the costimulatory CD28 receptor on T cells. We term these TSAxCD28 bispecifics and describe two such bispecific antibodies: one specific for …

The CD28 antibody recognizes the human CD28 antigen, a type I transmembrane protein, which is highly expressed on CD3 + thymocytes, most peripheral thymocytes and plasma cells. Binding of CD28 to its ligands CD80 or CD86 costimulates T cell effector function and T cell–dependent antibody production in vitro and in vivo .

2025年2月17日 · The CD64-CR is the major driver of the proliferation, expansion, and persistence of Fcγ-CR/CD28/CD3ζ T cells. (A) CD3-activated human T cells were transduced with CD16-CR, CD32-CR, or CD64-CR.The cell surface expression of Fcγ-CRs was evaluated over time utilizing specific PE-conjugated mouse anti-human FcγRs and FITC-conjugated mouse anti-human …