CT2A, CT2A-EGFRvIII, and HEK293 cells were graciously donated by Dr. John Sampson’s lab. CT2A and CT2A-EGFRvIII were modified to express GFP and/or luciferase by transducing lentivirus made by the ...

Metastatic breast cancer remains a major cause of cancer-related deaths in women, and there are few effective therapies against this advanced disease. Emerging evidence suggests that key steps of ...

Subsequently, GFP expressing cancer cells were sorted by flow cytometry using the FACS Aria III (BD biosciences). The presence of PIP in transduced 4T1 and E0771 cells was confirmed by Western blot ...

However, interactions between LN stroma and pioneer metastatic cells during metastatic colonization remain largely uncharacterized. Here we studied mice implanted with GFP-expressing melanoma cells to ...